Rapid blue light induction of protein interactions in living cells

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and...

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Autores principales: Kennedy, Matthew J., Hughes, Robert M., Peteya, Leslie A., Schwartz, Joel W., Ehlers, Michael D., Tucker, Chandra L.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059133/
https://www.ncbi.nlm.nih.gov/pubmed/21037589
http://dx.doi.org/10.1038/nmeth.1524
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author Kennedy, Matthew J.
Hughes, Robert M.
Peteya, Leslie A.
Schwartz, Joel W.
Ehlers, Michael D.
Tucker, Chandra L.
author_facet Kennedy, Matthew J.
Hughes, Robert M.
Peteya, Leslie A.
Schwartz, Joel W.
Ehlers, Michael D.
Tucker, Chandra L.
author_sort Kennedy, Matthew J.
collection PubMed
description Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue light exposure with sub-second time resolution and subcellular spatial resolution. We demonstrate the general utility of this system by inducing protein translocation, transcription, and Cre-mediated DNA recombination using light.
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spelling pubmed-30591332011-06-01 Rapid blue light induction of protein interactions in living cells Kennedy, Matthew J. Hughes, Robert M. Peteya, Leslie A. Schwartz, Joel W. Ehlers, Michael D. Tucker, Chandra L. Nat Methods Article Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue light exposure with sub-second time resolution and subcellular spatial resolution. We demonstrate the general utility of this system by inducing protein translocation, transcription, and Cre-mediated DNA recombination using light. 2010-10-31 2010-12 /pmc/articles/PMC3059133/ /pubmed/21037589 http://dx.doi.org/10.1038/nmeth.1524 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kennedy, Matthew J.
Hughes, Robert M.
Peteya, Leslie A.
Schwartz, Joel W.
Ehlers, Michael D.
Tucker, Chandra L.
Rapid blue light induction of protein interactions in living cells
title Rapid blue light induction of protein interactions in living cells
title_full Rapid blue light induction of protein interactions in living cells
title_fullStr Rapid blue light induction of protein interactions in living cells
title_full_unstemmed Rapid blue light induction of protein interactions in living cells
title_short Rapid blue light induction of protein interactions in living cells
title_sort rapid blue light induction of protein interactions in living cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059133/
https://www.ncbi.nlm.nih.gov/pubmed/21037589
http://dx.doi.org/10.1038/nmeth.1524
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AT schwartzjoelw rapidbluelightinductionofproteininteractionsinlivingcells
AT ehlersmichaeld rapidbluelightinductionofproteininteractionsinlivingcells
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