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Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential?
PURPOSE: Vitrification technology presents new opportunities for preservation of embryo derived stem cells without first establishing a viable ESC line. This study tests the feasibility of cryopreserving ICM cells using vitrification. MATERIALS AND METHODS: ICMs from mouse embryos were isolated and...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer US
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059526/ https://www.ncbi.nlm.nih.gov/pubmed/21057976 http://dx.doi.org/10.1007/s10815-010-9500-x |
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author | Desai, Nina Xu, Jing Tsulaia, Tamara Szeptycki-Lawson, Julia AbdelHafez, Faten Goldfarb, James Falcone, Tommaso |
author_facet | Desai, Nina Xu, Jing Tsulaia, Tamara Szeptycki-Lawson, Julia AbdelHafez, Faten Goldfarb, James Falcone, Tommaso |
author_sort | Desai, Nina |
collection | PubMed |
description | PURPOSE: Vitrification technology presents new opportunities for preservation of embryo derived stem cells without first establishing a viable ESC line. This study tests the feasibility of cryopreserving ICM cells using vitrification. MATERIALS AND METHODS: ICMs from mouse embryos were isolated and vitrified in HSV straws or on cryoloops. Upon warming, the vitrified ICMs were cultured and observed for attachment and morphology. Colonies were passaged every 3–6 days. ICMs and ICM-derived ESC colonies were tested for expression of stem cell specific markers. RESULTS: ICMs vitrified on both the cryoloop and the HSV straw had high survival rates. ICM derived ESCs remained undifferentiated for several passages and demonstrated expression of typical stem cell markers; SSEA-1, Sox-2, Oct 4 and alkaline phosphatase. CONCLUSION: This is the first report on successful vitrification of isolated ICMs and the subsequent derivation of ESC colonies. Vitrification of isolated ICMs is a novel approach for preservation of the “stem cell source” material. |
format | Text |
id | pubmed-3059526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-30595262011-04-05 Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? Desai, Nina Xu, Jing Tsulaia, Tamara Szeptycki-Lawson, Julia AbdelHafez, Faten Goldfarb, James Falcone, Tommaso J Assist Reprod Genet Stem Cell Biology PURPOSE: Vitrification technology presents new opportunities for preservation of embryo derived stem cells without first establishing a viable ESC line. This study tests the feasibility of cryopreserving ICM cells using vitrification. MATERIALS AND METHODS: ICMs from mouse embryos were isolated and vitrified in HSV straws or on cryoloops. Upon warming, the vitrified ICMs were cultured and observed for attachment and morphology. Colonies were passaged every 3–6 days. ICMs and ICM-derived ESC colonies were tested for expression of stem cell specific markers. RESULTS: ICMs vitrified on both the cryoloop and the HSV straw had high survival rates. ICM derived ESCs remained undifferentiated for several passages and demonstrated expression of typical stem cell markers; SSEA-1, Sox-2, Oct 4 and alkaline phosphatase. CONCLUSION: This is the first report on successful vitrification of isolated ICMs and the subsequent derivation of ESC colonies. Vitrification of isolated ICMs is a novel approach for preservation of the “stem cell source” material. Springer US 2010-11-06 2011-02 /pmc/articles/PMC3059526/ /pubmed/21057976 http://dx.doi.org/10.1007/s10815-010-9500-x Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Stem Cell Biology Desai, Nina Xu, Jing Tsulaia, Tamara Szeptycki-Lawson, Julia AbdelHafez, Faten Goldfarb, James Falcone, Tommaso Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? |
title | Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? |
title_full | Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? |
title_fullStr | Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? |
title_full_unstemmed | Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? |
title_short | Vitrification of mouse embryo-derived ICM cells: a tool for preserving embryonic stem cell potential? |
title_sort | vitrification of mouse embryo-derived icm cells: a tool for preserving embryonic stem cell potential? |
topic | Stem Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059526/ https://www.ncbi.nlm.nih.gov/pubmed/21057976 http://dx.doi.org/10.1007/s10815-010-9500-x |
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