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Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG
In this work we investigate multistability in the lac operon of Escherichia coli when it is induced by a mixture of lactose and the non-metabolizable thiomethyl galactoside (TMG). In accordance with previously published experimental results and computer simulations, our simulations predict that: (1)...
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Formato: | Texto |
Lenguaje: | English |
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Frontiers Research Foundation
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059962/ https://www.ncbi.nlm.nih.gov/pubmed/21423364 http://dx.doi.org/10.3389/fphys.2010.00022 |
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author | Díaz-Hernández, Orlando Santillán, Moisés |
author_facet | Díaz-Hernández, Orlando Santillán, Moisés |
author_sort | Díaz-Hernández, Orlando |
collection | PubMed |
description | In this work we investigate multistability in the lac operon of Escherichia coli when it is induced by a mixture of lactose and the non-metabolizable thiomethyl galactoside (TMG). In accordance with previously published experimental results and computer simulations, our simulations predict that: (1) when the system is induced by TMG, the system shows a discernible bistable behavior while, (2) when the system is induced by lactose, bistability does not disappear but excessively high concentrations of lactose would be required to observe it. Finally, our simulation results predict that when a mixture of lactose and TMG is used, the bistability region in the extracellular glucose concentration vs. extracellular lactose concentration parameter space changes in such a way that the model predictions regarding bistability could be tested experimentally. These experiments could help to solve a recent controversy regarding the existence of bistability in the lac operon under natural conditions. |
format | Text |
id | pubmed-3059962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Frontiers Research Foundation |
record_format | MEDLINE/PubMed |
spelling | pubmed-30599622011-03-21 Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG Díaz-Hernández, Orlando Santillán, Moisés Front Physiol Physiology In this work we investigate multistability in the lac operon of Escherichia coli when it is induced by a mixture of lactose and the non-metabolizable thiomethyl galactoside (TMG). In accordance with previously published experimental results and computer simulations, our simulations predict that: (1) when the system is induced by TMG, the system shows a discernible bistable behavior while, (2) when the system is induced by lactose, bistability does not disappear but excessively high concentrations of lactose would be required to observe it. Finally, our simulation results predict that when a mixture of lactose and TMG is used, the bistability region in the extracellular glucose concentration vs. extracellular lactose concentration parameter space changes in such a way that the model predictions regarding bistability could be tested experimentally. These experiments could help to solve a recent controversy regarding the existence of bistability in the lac operon under natural conditions. Frontiers Research Foundation 2010-07-19 /pmc/articles/PMC3059962/ /pubmed/21423364 http://dx.doi.org/10.3389/fphys.2010.00022 Text en Copyright © 2010 Díaz-Hernández and Santillán. http://www.frontiersin.org/licenseagreement This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited. |
spellingShingle | Physiology Díaz-Hernández, Orlando Santillán, Moisés Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG |
title | Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG |
title_full | Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG |
title_fullStr | Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG |
title_full_unstemmed | Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG |
title_short | Bistable Behavior of the Lac Operon in E. Coli When Induced with a Mixture of Lactose and TMG |
title_sort | bistable behavior of the lac operon in e. coli when induced with a mixture of lactose and tmg |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3059962/ https://www.ncbi.nlm.nih.gov/pubmed/21423364 http://dx.doi.org/10.3389/fphys.2010.00022 |
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