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Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes

BACKGROUND: Carbonic anhydrase (CA) of the chicken has attracted attention for a long time because it has an important role in the eggshell formation. The developmental profile of CA-II isozyme levels in chicken erythrocytes has not been determined or reported. Furthermore, the relations with CA-II...

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Autores principales: Nishita, Toshiho, Tomita, Yuichiro, Imanari, Takao, Ichihara, Nobutsune, Orito, Kensuke, Arishima, Kazuyoshi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060127/
https://www.ncbi.nlm.nih.gov/pubmed/21375785
http://dx.doi.org/10.1186/1751-0147-53-16
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author Nishita, Toshiho
Tomita, Yuichiro
Imanari, Takao
Ichihara, Nobutsune
Orito, Kensuke
Arishima, Kazuyoshi
author_facet Nishita, Toshiho
Tomita, Yuichiro
Imanari, Takao
Ichihara, Nobutsune
Orito, Kensuke
Arishima, Kazuyoshi
author_sort Nishita, Toshiho
collection PubMed
description BACKGROUND: Carbonic anhydrase (CA) of the chicken has attracted attention for a long time because it has an important role in the eggshell formation. The developmental profile of CA-II isozyme levels in chicken erythrocytes has not been determined or reported. Furthermore, the relations with CA-II in erythrocyte and egg production are not discussed. In the present study, we isolated CA-II from erythrocytes of chickens and determined age-related changes of CA-II levels in erythrocytes. METHODS: Chicken CA-II was purified by a combination of column chromatography. The levels of CA-II in the hemolysate of the chicken were determined using the ELISA system in blood samples from 279 female chickens, ages 1 to 93 weeks, 69 male chickens, ages 3 to 59 weeks and 52 weeks female Araucana-chickens. RESULTS: The mean concentration of CA-II in hemolysate from 1-week-old female was 50.8 ± 11.9 mg/g of Hb. The mean levels of CA-II in 25-week-old (188.1 ± 82.6 mg/g of Hb), 31-week-old (193.6 ± 69.7 mg/g of Hb) and 49-week-old (203.8 ± 123.5 mg/g of Hb) female-chickens showed the highest level of CA-II. The levels of CA-II in female WL-chickens significantly decreased at 63 week (139.0 ± 19.3 mg/g of Hb). The levels of CA-II in female WL-chicken did not change from week 63 until week 93.The mean level of CA-II in hemolysate of 3-week-old male WL-chickens was 78.3 ± 20.7 mg/g of Hb. The levels of CA-II in male WL-chickens did not show changes in the week 3 to week 59 timeframe. The mean level of CA-II in 53-week-old female Araucana-chickens was 23.4 ± 1.78 mg/g of Hb. These levels of CA-II were about 11% of those of 49-week-old female WL-chickens. Simple linear regression analysis showed significant associations between the level of CA-II and egg laying rate from 16 week-old at 63 week-old WL-chicken (p < 0.01). CONCLUSIONS: Developmental changes and sexual differences of CA-II concentration in WL-chicken erythrocytes were observed. The concentration of CA-II in the erythrocyte of WL-chicken was much higher than that in Araucana-chicken (p < 0.01).
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spelling pubmed-30601272011-03-18 Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes Nishita, Toshiho Tomita, Yuichiro Imanari, Takao Ichihara, Nobutsune Orito, Kensuke Arishima, Kazuyoshi Acta Vet Scand Research BACKGROUND: Carbonic anhydrase (CA) of the chicken has attracted attention for a long time because it has an important role in the eggshell formation. The developmental profile of CA-II isozyme levels in chicken erythrocytes has not been determined or reported. Furthermore, the relations with CA-II in erythrocyte and egg production are not discussed. In the present study, we isolated CA-II from erythrocytes of chickens and determined age-related changes of CA-II levels in erythrocytes. METHODS: Chicken CA-II was purified by a combination of column chromatography. The levels of CA-II in the hemolysate of the chicken were determined using the ELISA system in blood samples from 279 female chickens, ages 1 to 93 weeks, 69 male chickens, ages 3 to 59 weeks and 52 weeks female Araucana-chickens. RESULTS: The mean concentration of CA-II in hemolysate from 1-week-old female was 50.8 ± 11.9 mg/g of Hb. The mean levels of CA-II in 25-week-old (188.1 ± 82.6 mg/g of Hb), 31-week-old (193.6 ± 69.7 mg/g of Hb) and 49-week-old (203.8 ± 123.5 mg/g of Hb) female-chickens showed the highest level of CA-II. The levels of CA-II in female WL-chickens significantly decreased at 63 week (139.0 ± 19.3 mg/g of Hb). The levels of CA-II in female WL-chicken did not change from week 63 until week 93.The mean level of CA-II in hemolysate of 3-week-old male WL-chickens was 78.3 ± 20.7 mg/g of Hb. The levels of CA-II in male WL-chickens did not show changes in the week 3 to week 59 timeframe. The mean level of CA-II in 53-week-old female Araucana-chickens was 23.4 ± 1.78 mg/g of Hb. These levels of CA-II were about 11% of those of 49-week-old female WL-chickens. Simple linear regression analysis showed significant associations between the level of CA-II and egg laying rate from 16 week-old at 63 week-old WL-chicken (p < 0.01). CONCLUSIONS: Developmental changes and sexual differences of CA-II concentration in WL-chicken erythrocytes were observed. The concentration of CA-II in the erythrocyte of WL-chicken was much higher than that in Araucana-chicken (p < 0.01). BioMed Central 2011-03-07 /pmc/articles/PMC3060127/ /pubmed/21375785 http://dx.doi.org/10.1186/1751-0147-53-16 Text en Copyright ©2011 Nishita et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Nishita, Toshiho
Tomita, Yuichiro
Imanari, Takao
Ichihara, Nobutsune
Orito, Kensuke
Arishima, Kazuyoshi
Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
title Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
title_full Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
title_fullStr Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
title_full_unstemmed Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
title_short Biochemical and developmental characterization of carbonic anhydrase II from chicken erythrocytes
title_sort biochemical and developmental characterization of carbonic anhydrase ii from chicken erythrocytes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060127/
https://www.ncbi.nlm.nih.gov/pubmed/21375785
http://dx.doi.org/10.1186/1751-0147-53-16
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