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Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc

Stable isotope signatures of Zn have shown great promise in elucidating changes in uptake and translocation mechanisms of this metal in plants during environmental changes. Here this potential was tested by investigating the effect of high Zn concentrations on the isotopic fractionation patterns of...

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Autores principales: Caldelas, Cristina, Dong, Shuofei, Araus, José Luis, Jakob Weiss, Dominik
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060695/
https://www.ncbi.nlm.nih.gov/pubmed/21193582
http://dx.doi.org/10.1093/jxb/erq414
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author Caldelas, Cristina
Dong, Shuofei
Araus, José Luis
Jakob Weiss, Dominik
author_facet Caldelas, Cristina
Dong, Shuofei
Araus, José Luis
Jakob Weiss, Dominik
author_sort Caldelas, Cristina
collection PubMed
description Stable isotope signatures of Zn have shown great promise in elucidating changes in uptake and translocation mechanisms of this metal in plants during environmental changes. Here this potential was tested by investigating the effect of high Zn concentrations on the isotopic fractionation patterns of Phragmites australis (Cav.) Trin. ex Steud. Plants were grown for 40 d in a nutritive solution containing 3.2 μM (sufficient) or 2 mM (toxic) Zn. The Zn isotopic composition of roots, rhizomes, shoots, and leaves was analysed. Stems and leaves were sampled at different heights to evaluate the effect of long-distance transport on Zn fractionation. During Zn sufficiency, roots, rhizomes, and shoots were isotopically heavy (δ(66)Zn(JMC Lyon)=0.2‰) while the youngest leaves were isotopically light (–0.5‰). During Zn excess, roots were still isotopically heavier (δ(66)Zn=0.5‰) and the rest of the plant was isotopically light (up to –0.5‰). The enrichment of heavy isotopes at the roots was attributed to Zn uptake mediated by transporter proteins under Zn-sufficient conditions and to chelation and compartmentation in Zn excess. The isotopically lighter Zn in shoots and leaves is consistent with long-distance root to shoot transport. The tolerance response of P. australis increased the range of Zn fractionation within the plant and with respect to the environment.
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spelling pubmed-30606952011-03-18 Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc Caldelas, Cristina Dong, Shuofei Araus, José Luis Jakob Weiss, Dominik J Exp Bot Research Papers Stable isotope signatures of Zn have shown great promise in elucidating changes in uptake and translocation mechanisms of this metal in plants during environmental changes. Here this potential was tested by investigating the effect of high Zn concentrations on the isotopic fractionation patterns of Phragmites australis (Cav.) Trin. ex Steud. Plants were grown for 40 d in a nutritive solution containing 3.2 μM (sufficient) or 2 mM (toxic) Zn. The Zn isotopic composition of roots, rhizomes, shoots, and leaves was analysed. Stems and leaves were sampled at different heights to evaluate the effect of long-distance transport on Zn fractionation. During Zn sufficiency, roots, rhizomes, and shoots were isotopically heavy (δ(66)Zn(JMC Lyon)=0.2‰) while the youngest leaves were isotopically light (–0.5‰). During Zn excess, roots were still isotopically heavier (δ(66)Zn=0.5‰) and the rest of the plant was isotopically light (up to –0.5‰). The enrichment of heavy isotopes at the roots was attributed to Zn uptake mediated by transporter proteins under Zn-sufficient conditions and to chelation and compartmentation in Zn excess. The isotopically lighter Zn in shoots and leaves is consistent with long-distance root to shoot transport. The tolerance response of P. australis increased the range of Zn fractionation within the plant and with respect to the environment. Oxford University Press 2011-03 2010-12-30 /pmc/articles/PMC3060695/ /pubmed/21193582 http://dx.doi.org/10.1093/jxb/erq414 Text en © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Caldelas, Cristina
Dong, Shuofei
Araus, José Luis
Jakob Weiss, Dominik
Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
title Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
title_full Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
title_fullStr Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
title_full_unstemmed Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
title_short Zinc isotopic fractionation in Phragmites australis in response to toxic levels of zinc
title_sort zinc isotopic fractionation in phragmites australis in response to toxic levels of zinc
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060695/
https://www.ncbi.nlm.nih.gov/pubmed/21193582
http://dx.doi.org/10.1093/jxb/erq414
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