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ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport

The primary inflorescence stem of Arabidopsis thaliana is rich in lignified cell walls, in both vascular bundles and interfascicular fibres. Previous gene expression studies demonstrated a correlation between expression of phenylpropanoid biosynthetic genes and a subset of genes encoding ATP-binding...

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Autores principales: Kaneda, M., Schuetz, M., Lin, B.S.P., Chanis, C., Hamberger, B., Western, T.L., Ehlting, J., Samuels, A.L.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060696/
https://www.ncbi.nlm.nih.gov/pubmed/21239383
http://dx.doi.org/10.1093/jxb/erq416
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author Kaneda, M.
Schuetz, M.
Lin, B.S.P.
Chanis, C.
Hamberger, B.
Western, T.L.
Ehlting, J.
Samuels, A.L.
author_facet Kaneda, M.
Schuetz, M.
Lin, B.S.P.
Chanis, C.
Hamberger, B.
Western, T.L.
Ehlting, J.
Samuels, A.L.
author_sort Kaneda, M.
collection PubMed
description The primary inflorescence stem of Arabidopsis thaliana is rich in lignified cell walls, in both vascular bundles and interfascicular fibres. Previous gene expression studies demonstrated a correlation between expression of phenylpropanoid biosynthetic genes and a subset of genes encoding ATP-binding cassette (ABC) transporters, especially in the ABCB/multi-drug resistance/P-glycoprotein (ABCB/MDR/PGP) and ABCG/pleiotropic drug resistance (ABCG/PDR) subfamilies. The objective of this study was to characterize these ABC transporters in terms of their gene expression and their function in development of lignified cells. Based on in silico analyses, four ABC transporters were selected for detailed investigation: ABCB11/MDR8, ABCB14/MDR12, ABCB15/MDR13, and ABCG33/PDR5. Promoter::glucuronidase reporter assays for each gene indicated that promoters of ABCB11, ABCB14, ABCB15, and ABCG33 transporters are active in the vascular tissues of primary stem, and in some cases in interfascicular tissues as well. Homozygous T-DNA insertion mutant lines showed no apparent irregular xylem phenotype or alterations in interfascicular fibre lignification or morphology in comparison with wild type. However, in abcb14-1 mutants, stem vascular morphology was slightly disorganized, with decreased phloem area in the vascular bundle and decreased xylem vessel lumen diameter. In addition, abcb14-1 mutants showed both decreased polar auxin transport through whole stems and altered auxin distribution in the procambium. It is proposed that both ABCB14 and ABCB15 promote auxin transport since inflorescence stems in both mutants showed a reduction in polar auxin transport, which was not observed for any of the ABCG subfamily mutants tested. In the case of ABCB14, the reduction in auxin transport is correlated with a mild disruption of vascular development in the inflorescence stem.
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spelling pubmed-30606962011-03-18 ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport Kaneda, M. Schuetz, M. Lin, B.S.P. Chanis, C. Hamberger, B. Western, T.L. Ehlting, J. Samuels, A.L. J Exp Bot Research Papers The primary inflorescence stem of Arabidopsis thaliana is rich in lignified cell walls, in both vascular bundles and interfascicular fibres. Previous gene expression studies demonstrated a correlation between expression of phenylpropanoid biosynthetic genes and a subset of genes encoding ATP-binding cassette (ABC) transporters, especially in the ABCB/multi-drug resistance/P-glycoprotein (ABCB/MDR/PGP) and ABCG/pleiotropic drug resistance (ABCG/PDR) subfamilies. The objective of this study was to characterize these ABC transporters in terms of their gene expression and their function in development of lignified cells. Based on in silico analyses, four ABC transporters were selected for detailed investigation: ABCB11/MDR8, ABCB14/MDR12, ABCB15/MDR13, and ABCG33/PDR5. Promoter::glucuronidase reporter assays for each gene indicated that promoters of ABCB11, ABCB14, ABCB15, and ABCG33 transporters are active in the vascular tissues of primary stem, and in some cases in interfascicular tissues as well. Homozygous T-DNA insertion mutant lines showed no apparent irregular xylem phenotype or alterations in interfascicular fibre lignification or morphology in comparison with wild type. However, in abcb14-1 mutants, stem vascular morphology was slightly disorganized, with decreased phloem area in the vascular bundle and decreased xylem vessel lumen diameter. In addition, abcb14-1 mutants showed both decreased polar auxin transport through whole stems and altered auxin distribution in the procambium. It is proposed that both ABCB14 and ABCB15 promote auxin transport since inflorescence stems in both mutants showed a reduction in polar auxin transport, which was not observed for any of the ABCG subfamily mutants tested. In the case of ABCB14, the reduction in auxin transport is correlated with a mild disruption of vascular development in the inflorescence stem. Oxford University Press 2011-03 2011-01-14 /pmc/articles/PMC3060696/ /pubmed/21239383 http://dx.doi.org/10.1093/jxb/erq416 Text en © 2011 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Kaneda, M.
Schuetz, M.
Lin, B.S.P.
Chanis, C.
Hamberger, B.
Western, T.L.
Ehlting, J.
Samuels, A.L.
ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport
title ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport
title_full ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport
title_fullStr ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport
title_full_unstemmed ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport
title_short ABC transporters coordinately expressed during lignification of Arabidopsis stems include a set of ABCBs associated with auxin transport
title_sort abc transporters coordinately expressed during lignification of arabidopsis stems include a set of abcbs associated with auxin transport
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060696/
https://www.ncbi.nlm.nih.gov/pubmed/21239383
http://dx.doi.org/10.1093/jxb/erq416
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