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Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family
Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease associated with a significant mortality rate. Type III secreted effectors are major contributors to the pathogenesis of Salmonella infections...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060822/ https://www.ncbi.nlm.nih.gov/pubmed/21445262 http://dx.doi.org/10.1371/journal.pone.0017824 |
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author | Brown, Nat F. Coombes, Brian K. Bishop, Jenna L. Wickham, Mark E. Lowden, Michael J. Gal-Mor, Ohad Goode, David L. Boyle, Erin C. Sanderson, Kristy L. Finlay, B. Brett |
author_facet | Brown, Nat F. Coombes, Brian K. Bishop, Jenna L. Wickham, Mark E. Lowden, Michael J. Gal-Mor, Ohad Goode, David L. Boyle, Erin C. Sanderson, Kristy L. Finlay, B. Brett |
author_sort | Brown, Nat F. |
collection | PubMed |
description | Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease associated with a significant mortality rate. Type III secreted effectors are major contributors to the pathogenesis of Salmonella infections. Genes encoding effectors are acquired via horizontal gene transfer, and a subset are encoded within active phage lysogens. Because the acquisition of effectors is in flux, the complement of effectors possessed by various Salmonella strains frequently differs. By comparing the genome sequences of S. enterica serovar Typhimurium strain SL1344 with LT2, we identified a gene with significant similarity to SseK/NleB type III secreted effector proteins within a phage ST64B lysogen that is absent from LT2. We have named this gene sseK3. SseK3 was co-regulated with the SPI-2 type III secretion system in vitro and inside host cells, and was also injected into infected host cells. While no role for SseK3 in virulence could be identified, a role for the other family members in murine typhoid was found. SseK3 and other phage-encoded effectors were found to have a significant but sparse distribution in the available Salmonella genome sequences, indicating the potential for more uncharacterised effectors to be present in less studied serovars. These phage-encoded effectors may be principle subjects of contemporary selective processes shaping Salmonella-host interactions. |
format | Text |
id | pubmed-3060822 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30608222011-03-28 Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family Brown, Nat F. Coombes, Brian K. Bishop, Jenna L. Wickham, Mark E. Lowden, Michael J. Gal-Mor, Ohad Goode, David L. Boyle, Erin C. Sanderson, Kristy L. Finlay, B. Brett PLoS One Research Article Salmonella enterica is a species of bacteria that is a major cause of enteritis across the globe, while certain serovars cause typhoid, a more serious disease associated with a significant mortality rate. Type III secreted effectors are major contributors to the pathogenesis of Salmonella infections. Genes encoding effectors are acquired via horizontal gene transfer, and a subset are encoded within active phage lysogens. Because the acquisition of effectors is in flux, the complement of effectors possessed by various Salmonella strains frequently differs. By comparing the genome sequences of S. enterica serovar Typhimurium strain SL1344 with LT2, we identified a gene with significant similarity to SseK/NleB type III secreted effector proteins within a phage ST64B lysogen that is absent from LT2. We have named this gene sseK3. SseK3 was co-regulated with the SPI-2 type III secretion system in vitro and inside host cells, and was also injected into infected host cells. While no role for SseK3 in virulence could be identified, a role for the other family members in murine typhoid was found. SseK3 and other phage-encoded effectors were found to have a significant but sparse distribution in the available Salmonella genome sequences, indicating the potential for more uncharacterised effectors to be present in less studied serovars. These phage-encoded effectors may be principle subjects of contemporary selective processes shaping Salmonella-host interactions. Public Library of Science 2011-03-18 /pmc/articles/PMC3060822/ /pubmed/21445262 http://dx.doi.org/10.1371/journal.pone.0017824 Text en Brown et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Brown, Nat F. Coombes, Brian K. Bishop, Jenna L. Wickham, Mark E. Lowden, Michael J. Gal-Mor, Ohad Goode, David L. Boyle, Erin C. Sanderson, Kristy L. Finlay, B. Brett Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family |
title |
Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family |
title_full |
Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family |
title_fullStr |
Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family |
title_full_unstemmed |
Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family |
title_short |
Salmonella Phage ST64B Encodes a Member of the SseK/NleB Effector Family |
title_sort | salmonella phage st64b encodes a member of the ssek/nleb effector family |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060822/ https://www.ncbi.nlm.nih.gov/pubmed/21445262 http://dx.doi.org/10.1371/journal.pone.0017824 |
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