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Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions
It has been generally recognized that understanding the molecular basis of some important cellular processes is hampered by the lack of knowledge of forces that drive spontaneous formation/disruption of G-quadruplex structures in guanine-rich DNA sequences. According to numerous biophysical and stru...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061076/ https://www.ncbi.nlm.nih.gov/pubmed/21113023 http://dx.doi.org/10.1093/nar/gkq867 |
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author | Prislan, Iztok Lah, Jurij Milanic, Matija Vesnaver, Gorazd |
author_facet | Prislan, Iztok Lah, Jurij Milanic, Matija Vesnaver, Gorazd |
author_sort | Prislan, Iztok |
collection | PubMed |
description | It has been generally recognized that understanding the molecular basis of some important cellular processes is hampered by the lack of knowledge of forces that drive spontaneous formation/disruption of G-quadruplex structures in guanine-rich DNA sequences. According to numerous biophysical and structural studies G-quadruplexes may occur in the presence of K(+) and Na(+) ions as polymorphic structures formed in kinetically governed processes. The reported kinetic models suggested to describe this polymorphism should be considered inappropriate since, as a rule, they include bimolecular single-step associations characterized by negative activation energies. In contrast, our approach in studying polymorphic behavior of G-quadruplexes is based on model mechanisms that involve only elementary folding/unfolding transitions and structural conversion steps that are characterized by positive activation energies. Here, we are investigating a complex polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions. On the basis of DSC, circular dichroism and UV spectroscopy and polyacrylamide gel electrophoresis experiments we propose a kinetic model that successfully describes the observed thermally induced conformational transitions of d(G(4)T(4)G(3)) quadruplexes in terms of single-step reactions that involve besides single strands also one tetramolecular and three bimolecular quadruplex structures. |
format | Text |
id | pubmed-3061076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-30610762011-03-21 Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions Prislan, Iztok Lah, Jurij Milanic, Matija Vesnaver, Gorazd Nucleic Acids Res Structural Biology It has been generally recognized that understanding the molecular basis of some important cellular processes is hampered by the lack of knowledge of forces that drive spontaneous formation/disruption of G-quadruplex structures in guanine-rich DNA sequences. According to numerous biophysical and structural studies G-quadruplexes may occur in the presence of K(+) and Na(+) ions as polymorphic structures formed in kinetically governed processes. The reported kinetic models suggested to describe this polymorphism should be considered inappropriate since, as a rule, they include bimolecular single-step associations characterized by negative activation energies. In contrast, our approach in studying polymorphic behavior of G-quadruplexes is based on model mechanisms that involve only elementary folding/unfolding transitions and structural conversion steps that are characterized by positive activation energies. Here, we are investigating a complex polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions. On the basis of DSC, circular dichroism and UV spectroscopy and polyacrylamide gel electrophoresis experiments we propose a kinetic model that successfully describes the observed thermally induced conformational transitions of d(G(4)T(4)G(3)) quadruplexes in terms of single-step reactions that involve besides single strands also one tetramolecular and three bimolecular quadruplex structures. Oxford University Press 2011-03 2010-11-26 /pmc/articles/PMC3061076/ /pubmed/21113023 http://dx.doi.org/10.1093/nar/gkq867 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Structural Biology Prislan, Iztok Lah, Jurij Milanic, Matija Vesnaver, Gorazd Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions |
title | Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions |
title_full | Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions |
title_fullStr | Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions |
title_full_unstemmed | Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions |
title_short | Kinetically governed polymorphism of d(G(4)T(4)G(3)) quadruplexes in K(+) solutions |
title_sort | kinetically governed polymorphism of d(g(4)t(4)g(3)) quadruplexes in k(+) solutions |
topic | Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061076/ https://www.ncbi.nlm.nih.gov/pubmed/21113023 http://dx.doi.org/10.1093/nar/gkq867 |
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