RNAi Screening for Fat Regulatory Genes with SRS Microscopy

Searching for genes regulating fat accumulation has both fundamental and medical interests. Genetic screening for those genes in Caenorhabditis elegans, a widely-used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging o...

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Detalles Bibliográficos
Autores principales: Wang, Meng C., Min, Wei, Freudiger, Christian W., Ruvkun, Gary, Xie, X. Sunney
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061290/
https://www.ncbi.nlm.nih.gov/pubmed/21240281
http://dx.doi.org/10.1038/nmeth.1556
Descripción
Sumario:Searching for genes regulating fat accumulation has both fundamental and medical interests. Genetic screening for those genes in Caenorhabditis elegans, a widely-used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering microscopy, which overcomes major limitations of coherent anti-stokes Raman scattering microscopy. Our screening yielded eight novel genetic regulators of fat storage.

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