RNAi Screening for Fat Regulatory Genes with SRS Microscopy

Searching for genes regulating fat accumulation has both fundamental and medical interests. Genetic screening for those genes in Caenorhabditis elegans, a widely-used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging o...

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Detalles Bibliográficos
Autores principales: Wang, Meng C., Min, Wei, Freudiger, Christian W., Ruvkun, Gary, Xie, X. Sunney
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061290/
https://www.ncbi.nlm.nih.gov/pubmed/21240281
http://dx.doi.org/10.1038/nmeth.1556
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author Wang, Meng C.
Min, Wei
Freudiger, Christian W.
Ruvkun, Gary
Xie, X. Sunney
author_facet Wang, Meng C.
Min, Wei
Freudiger, Christian W.
Ruvkun, Gary
Xie, X. Sunney
author_sort Wang, Meng C.
collection PubMed
description Searching for genes regulating fat accumulation has both fundamental and medical interests. Genetic screening for those genes in Caenorhabditis elegans, a widely-used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering microscopy, which overcomes major limitations of coherent anti-stokes Raman scattering microscopy. Our screening yielded eight novel genetic regulators of fat storage.
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spelling pubmed-30612902011-08-01 RNAi Screening for Fat Regulatory Genes with SRS Microscopy Wang, Meng C. Min, Wei Freudiger, Christian W. Ruvkun, Gary Xie, X. Sunney Nat Methods Article Searching for genes regulating fat accumulation has both fundamental and medical interests. Genetic screening for those genes in Caenorhabditis elegans, a widely-used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering microscopy, which overcomes major limitations of coherent anti-stokes Raman scattering microscopy. Our screening yielded eight novel genetic regulators of fat storage. 2011-01-16 2011-02 /pmc/articles/PMC3061290/ /pubmed/21240281 http://dx.doi.org/10.1038/nmeth.1556 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Meng C.
Min, Wei
Freudiger, Christian W.
Ruvkun, Gary
Xie, X. Sunney
RNAi Screening for Fat Regulatory Genes with SRS Microscopy
title RNAi Screening for Fat Regulatory Genes with SRS Microscopy
title_full RNAi Screening for Fat Regulatory Genes with SRS Microscopy
title_fullStr RNAi Screening for Fat Regulatory Genes with SRS Microscopy
title_full_unstemmed RNAi Screening for Fat Regulatory Genes with SRS Microscopy
title_short RNAi Screening for Fat Regulatory Genes with SRS Microscopy
title_sort rnai screening for fat regulatory genes with srs microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061290/
https://www.ncbi.nlm.nih.gov/pubmed/21240281
http://dx.doi.org/10.1038/nmeth.1556
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