Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus

The aromatic β-carboline norharmane was determined in workers, nymphs, and ergatoids of Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae) by gas chromatography/mass spectrometry. Norharmane levels in workers, nymphs, and ergatoids collected in May (∼4.3 ng/termite) were higher than those i...

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Detalles Bibliográficos
Autores principales: Itakura, Shuji, Kawabata, Satoshi, Tanaka, Hiromi, Enoki, Akio
Formato: Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061585/
https://www.ncbi.nlm.nih.gov/pubmed/20345286
http://dx.doi.org/10.1673/031.008.1301
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author Itakura, Shuji
Kawabata, Satoshi
Tanaka, Hiromi
Enoki, Akio
author_facet Itakura, Shuji
Kawabata, Satoshi
Tanaka, Hiromi
Enoki, Akio
author_sort Itakura, Shuji
collection PubMed
description The aromatic β-carboline norharmane was determined in workers, nymphs, and ergatoids of Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae) by gas chromatography/mass spectrometry. Norharmane levels in workers, nymphs, and ergatoids collected in May (∼4.3 ng/termite) were higher than those in November (∼0.2 ng/termite). Fluorescence of norharmane was observed in histological sections in whole animals and in the fat body. Norharmane, at a final concentration of 1 mM, stimulated juvenile hormone epoxide hydrolase (JHEH) activity of enzyme extract from ergatoids, but inhibited JHEH activity at higher concentrations. The elevated JHEH activity stimulated by norharmane should accelerate juvenile hormone metabolism in R. speratus.
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spelling pubmed-30615852011-07-21 Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus Itakura, Shuji Kawabata, Satoshi Tanaka, Hiromi Enoki, Akio J Insect Sci Article The aromatic β-carboline norharmane was determined in workers, nymphs, and ergatoids of Reticulitermes speratus (Kolbe) (Isoptera: Rhinotermitidae) by gas chromatography/mass spectrometry. Norharmane levels in workers, nymphs, and ergatoids collected in May (∼4.3 ng/termite) were higher than those in November (∼0.2 ng/termite). Fluorescence of norharmane was observed in histological sections in whole animals and in the fat body. Norharmane, at a final concentration of 1 mM, stimulated juvenile hormone epoxide hydrolase (JHEH) activity of enzyme extract from ergatoids, but inhibited JHEH activity at higher concentrations. The elevated JHEH activity stimulated by norharmane should accelerate juvenile hormone metabolism in R. speratus. University of Wisconsin Library 2008-02-21 /pmc/articles/PMC3061585/ /pubmed/20345286 http://dx.doi.org/10.1673/031.008.1301 Text en http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Itakura, Shuji
Kawabata, Satoshi
Tanaka, Hiromi
Enoki, Akio
Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus
title Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus
title_full Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus
title_fullStr Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus
title_full_unstemmed Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus
title_short Effect of Norharmane in vitro on Juvenile Hormone Epoxide Hydrolase Activity in the Lower Termite, Reticulitermes speratus
title_sort effect of norharmane in vitro on juvenile hormone epoxide hydrolase activity in the lower termite, reticulitermes speratus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061585/
https://www.ncbi.nlm.nih.gov/pubmed/20345286
http://dx.doi.org/10.1673/031.008.1301
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AT tanakahiromi effectofnorharmaneinvitroonjuvenilehormoneepoxidehydrolaseactivityinthelowertermitereticulitermessperatus
AT enokiakio effectofnorharmaneinvitroonjuvenilehormoneepoxidehydrolaseactivityinthelowertermitereticulitermessperatus

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