Segregation of European Corn Borer, Ostrinia nubilalis, Aminopeptidase 1, Cadherin, and Bre5-Like Alleles, from a Colony Resistant to Bacillus thuringiensis Cry1Ab Toxins, are not Associated with F(2) Larval Weights when Fed a Diet Containing Cry1Ab

Protein receptors may be required for activated Bacillus thuringiensis Cry toxins (Cry1Ab) to bind midgut epithelium prior to pore formation. Single nucleotide polymorphism markers from two Ostrinia nubilalis Hübner (Lepidoptera: Crambidae) midgut peptide receptors, cadherin (OnCad), aminopeptidase N 1 (OnAPN1), and OnBre5 (Onb3GalT5; a β-1,3-galactosyltransferase family 5 member) were used to examine segregation in F(2) families derived from paired matings of Cry1Ab-resistant females and Cry1Ab-susceptible males. Genotypic frequencies for these markers did not deviate from Mendelian expectations. Analysis of F(2) larvae indicate the segregation of single nucleotide pores in OnAPN1, OnBre5 (Onb3GalT5), and OnCad marker loci were independent of the segregation of logio weights of larvae feeding on Cry1Ab diet.