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Effects of Selenium Supplementation on Selenoprotein Gene Expression and Response to Influenza Vaccine Challenge: A Randomised Controlled Trial
BACKGROUND: The uncertainty surrounding dietary requirements for selenium (Se) is partly due to limitations in biomarkers of Se status that are related to health outcomes. In this study we determined the effect of different doses and forms of Se on gene expression of selenoprotein S (SEPS1), selenop...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061857/ https://www.ncbi.nlm.nih.gov/pubmed/21445287 http://dx.doi.org/10.1371/journal.pone.0014771 |
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author | Goldson, Andrew J. Fairweather-Tait, Susan J. Armah, Charlotte N. Bao, Yongping Broadley, Martin R. Dainty, Jack R. Furniss, Caroline Hart, David J. Teucher, Birgit Hurst, Rachel |
author_facet | Goldson, Andrew J. Fairweather-Tait, Susan J. Armah, Charlotte N. Bao, Yongping Broadley, Martin R. Dainty, Jack R. Furniss, Caroline Hart, David J. Teucher, Birgit Hurst, Rachel |
author_sort | Goldson, Andrew J. |
collection | PubMed |
description | BACKGROUND: The uncertainty surrounding dietary requirements for selenium (Se) is partly due to limitations in biomarkers of Se status that are related to health outcomes. In this study we determined the effect of different doses and forms of Se on gene expression of selenoprotein S (SEPS1), selenoprotein W (SEPW1) and selenoprotein R (SEPR), and responses to an immune function challenge, influenza vaccine, were measured in order to identify functional markers of Se status. METHODS AND FINDINGS: A 12 week human dietary intervention study was undertaken in 119 volunteers who received placebo, 50, 100 or 200 µg/day Se-enriched yeast (Se-yeast) or meals containing unenriched or Se-enriched onions (50 µg/day). Gene expression was quantified in RNA samples extracted from human peripheral blood mononuclear cells (PBMC's) using quantitative RT-PCR. There was a significant increase in SEPW1 mRNA in the Se-enriched onion group (50 µg/day) compared with the unenriched onion group. SEPR and SEPW1 did not change significantly over the duration of the supplementation period in the control or Se-yeast groups, except at week 10 when SEPW1 mRNA levels were significantly lower in the 200 µg/day Se-yeast group compared to the placebo group. Levels of SEPS1 mRNA increased significantly 7 days after the influenza vaccine challenge, the magnitude of the increase in SEPS1 gene expression was dose-dependent, with a significantly greater response with higher Se supplementation. CONCLUSIONS: This novel finding provides preliminary evidence for a role of SEPS1 in the immune response, and further supports the relationship between Se status and immune function. TRIAL REGISTRATION: ClinicalTrials.gov [NCT00279812] |
format | Text |
id | pubmed-3061857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30618572011-03-28 Effects of Selenium Supplementation on Selenoprotein Gene Expression and Response to Influenza Vaccine Challenge: A Randomised Controlled Trial Goldson, Andrew J. Fairweather-Tait, Susan J. Armah, Charlotte N. Bao, Yongping Broadley, Martin R. Dainty, Jack R. Furniss, Caroline Hart, David J. Teucher, Birgit Hurst, Rachel PLoS One Research Article BACKGROUND: The uncertainty surrounding dietary requirements for selenium (Se) is partly due to limitations in biomarkers of Se status that are related to health outcomes. In this study we determined the effect of different doses and forms of Se on gene expression of selenoprotein S (SEPS1), selenoprotein W (SEPW1) and selenoprotein R (SEPR), and responses to an immune function challenge, influenza vaccine, were measured in order to identify functional markers of Se status. METHODS AND FINDINGS: A 12 week human dietary intervention study was undertaken in 119 volunteers who received placebo, 50, 100 or 200 µg/day Se-enriched yeast (Se-yeast) or meals containing unenriched or Se-enriched onions (50 µg/day). Gene expression was quantified in RNA samples extracted from human peripheral blood mononuclear cells (PBMC's) using quantitative RT-PCR. There was a significant increase in SEPW1 mRNA in the Se-enriched onion group (50 µg/day) compared with the unenriched onion group. SEPR and SEPW1 did not change significantly over the duration of the supplementation period in the control or Se-yeast groups, except at week 10 when SEPW1 mRNA levels were significantly lower in the 200 µg/day Se-yeast group compared to the placebo group. Levels of SEPS1 mRNA increased significantly 7 days after the influenza vaccine challenge, the magnitude of the increase in SEPS1 gene expression was dose-dependent, with a significantly greater response with higher Se supplementation. CONCLUSIONS: This novel finding provides preliminary evidence for a role of SEPS1 in the immune response, and further supports the relationship between Se status and immune function. TRIAL REGISTRATION: ClinicalTrials.gov [NCT00279812] Public Library of Science 2011-03-21 /pmc/articles/PMC3061857/ /pubmed/21445287 http://dx.doi.org/10.1371/journal.pone.0014771 Text en Goldson et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Goldson, Andrew J. Fairweather-Tait, Susan J. Armah, Charlotte N. Bao, Yongping Broadley, Martin R. Dainty, Jack R. Furniss, Caroline Hart, David J. Teucher, Birgit Hurst, Rachel Effects of Selenium Supplementation on Selenoprotein Gene Expression and Response to Influenza Vaccine Challenge: A Randomised Controlled Trial |
title | Effects of Selenium Supplementation on Selenoprotein Gene Expression
and Response to Influenza Vaccine Challenge: A Randomised Controlled
Trial |
title_full | Effects of Selenium Supplementation on Selenoprotein Gene Expression
and Response to Influenza Vaccine Challenge: A Randomised Controlled
Trial |
title_fullStr | Effects of Selenium Supplementation on Selenoprotein Gene Expression
and Response to Influenza Vaccine Challenge: A Randomised Controlled
Trial |
title_full_unstemmed | Effects of Selenium Supplementation on Selenoprotein Gene Expression
and Response to Influenza Vaccine Challenge: A Randomised Controlled
Trial |
title_short | Effects of Selenium Supplementation on Selenoprotein Gene Expression
and Response to Influenza Vaccine Challenge: A Randomised Controlled
Trial |
title_sort | effects of selenium supplementation on selenoprotein gene expression
and response to influenza vaccine challenge: a randomised controlled
trial |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061857/ https://www.ncbi.nlm.nih.gov/pubmed/21445287 http://dx.doi.org/10.1371/journal.pone.0014771 |
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