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A gastrointestinal rotavirus infection mouse model for immune modulation studies

BACKGROUND: Rotaviruses are the single most important cause of severe diarrhea in young children worldwide. The current study was conducted to assess whether colostrum containing rotavirus-specific antibodies (Gastrogard-R(®)) could protect against rotavirus infection. In addition, this illness mode...

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Autores principales: Knipping, Karen, McNeal, Monica M, Crienen, Annelies, van Amerongen, Geert, Garssen, Johan, van't Land, Belinda
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061940/
https://www.ncbi.nlm.nih.gov/pubmed/21385425
http://dx.doi.org/10.1186/1743-422X-8-109
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author Knipping, Karen
McNeal, Monica M
Crienen, Annelies
van Amerongen, Geert
Garssen, Johan
van't Land, Belinda
author_facet Knipping, Karen
McNeal, Monica M
Crienen, Annelies
van Amerongen, Geert
Garssen, Johan
van't Land, Belinda
author_sort Knipping, Karen
collection PubMed
description BACKGROUND: Rotaviruses are the single most important cause of severe diarrhea in young children worldwide. The current study was conducted to assess whether colostrum containing rotavirus-specific antibodies (Gastrogard-R(®)) could protect against rotavirus infection. In addition, this illness model was used to study modulatory effects of intervention on several immune parameters after re-infection. METHODS: BALB/c mice were treated by gavage once daily with Gastrogard-R(® )from the age of 4 to 10 days, and were inoculated with rhesus rotavirus (RRV) at 7 days of age. A secondary inoculation with epizootic-diarrhea infant-mouse (EDIM) virus was administered at 17 days of age. Disease symptoms were scored daily and viral shedding was measured in fecal samples during the post-inoculation periods. Rotavirus-specific IgM, IgG and IgG subclasses in serum, T cell proliferation and rotavirus-specific delayed-type hypersensitivity (DTH) responses were also measured. RESULTS: Primary inoculation with RRV induced a mild but consistent level of diarrhea during 3-4 days post-inoculation. All mice receiving Gastrogard-R(® )were 100% protected against rotavirus-induced diarrhea. Mice receiving both RRV and EDIM inoculation had a lower faecal-viral load following EDIM inoculation then mice receiving EDIM alone or Gastrogard-R(®). Mice receiving Gastrogard-R(® )however displayed an enhanced rotavirus-specific T-cell proliferation whereas rotavirus-specific antibody subtypes were not affected. CONCLUSIONS: Preventing RRV-induced diarrhea by Gastrogard-R(® )early in life showed a diminished protection against EDIM re-infection, but a rotavirus-specific immune response was developed including both B cell and T cell responses. In general, this intervention model can be used for studying clinical symptoms as well as the immune responses required for protection against viral re-infection.
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spelling pubmed-30619402011-03-22 A gastrointestinal rotavirus infection mouse model for immune modulation studies Knipping, Karen McNeal, Monica M Crienen, Annelies van Amerongen, Geert Garssen, Johan van't Land, Belinda Virol J Research BACKGROUND: Rotaviruses are the single most important cause of severe diarrhea in young children worldwide. The current study was conducted to assess whether colostrum containing rotavirus-specific antibodies (Gastrogard-R(®)) could protect against rotavirus infection. In addition, this illness model was used to study modulatory effects of intervention on several immune parameters after re-infection. METHODS: BALB/c mice were treated by gavage once daily with Gastrogard-R(® )from the age of 4 to 10 days, and were inoculated with rhesus rotavirus (RRV) at 7 days of age. A secondary inoculation with epizootic-diarrhea infant-mouse (EDIM) virus was administered at 17 days of age. Disease symptoms were scored daily and viral shedding was measured in fecal samples during the post-inoculation periods. Rotavirus-specific IgM, IgG and IgG subclasses in serum, T cell proliferation and rotavirus-specific delayed-type hypersensitivity (DTH) responses were also measured. RESULTS: Primary inoculation with RRV induced a mild but consistent level of diarrhea during 3-4 days post-inoculation. All mice receiving Gastrogard-R(® )were 100% protected against rotavirus-induced diarrhea. Mice receiving both RRV and EDIM inoculation had a lower faecal-viral load following EDIM inoculation then mice receiving EDIM alone or Gastrogard-R(®). Mice receiving Gastrogard-R(® )however displayed an enhanced rotavirus-specific T-cell proliferation whereas rotavirus-specific antibody subtypes were not affected. CONCLUSIONS: Preventing RRV-induced diarrhea by Gastrogard-R(® )early in life showed a diminished protection against EDIM re-infection, but a rotavirus-specific immune response was developed including both B cell and T cell responses. In general, this intervention model can be used for studying clinical symptoms as well as the immune responses required for protection against viral re-infection. BioMed Central 2011-03-08 /pmc/articles/PMC3061940/ /pubmed/21385425 http://dx.doi.org/10.1186/1743-422X-8-109 Text en Copyright ©2011 Knipping et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Knipping, Karen
McNeal, Monica M
Crienen, Annelies
van Amerongen, Geert
Garssen, Johan
van't Land, Belinda
A gastrointestinal rotavirus infection mouse model for immune modulation studies
title A gastrointestinal rotavirus infection mouse model for immune modulation studies
title_full A gastrointestinal rotavirus infection mouse model for immune modulation studies
title_fullStr A gastrointestinal rotavirus infection mouse model for immune modulation studies
title_full_unstemmed A gastrointestinal rotavirus infection mouse model for immune modulation studies
title_short A gastrointestinal rotavirus infection mouse model for immune modulation studies
title_sort gastrointestinal rotavirus infection mouse model for immune modulation studies
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3061940/
https://www.ncbi.nlm.nih.gov/pubmed/21385425
http://dx.doi.org/10.1186/1743-422X-8-109
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