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Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori
A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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University of Wisconsin Library
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062493/ https://www.ncbi.nlm.nih.gov/pubmed/20331401 http://dx.doi.org/10.1673/031.008.5401 |
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author | Yan, Liu Zhi-Qi, Meng Bao-Long, Niu Li-Hua, He Hong-Biao, Weng Wei-Feng, Shen |
author_facet | Yan, Liu Zhi-Qi, Meng Bao-Long, Niu Li-Hua, He Hong-Biao, Weng Wei-Feng, Shen |
author_sort | Yan, Liu |
collection | PubMed |
description | A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that the B. mori tryptophan oxygenase gene was transcribed in all examined stages. Tryptophan oxygenase proteins are relatively well conserved among different orders of arthropods. |
format | Text |
id | pubmed-3062493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | University of Wisconsin Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-30624932011-07-21 Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori Yan, Liu Zhi-Qi, Meng Bao-Long, Niu Li-Hua, He Hong-Biao, Weng Wei-Feng, Shen J Insect Sci Article A Bombyx mori L. (Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. RT-PCR analysis showed that the B. mori tryptophan oxygenase gene was transcribed in all examined stages. Tryptophan oxygenase proteins are relatively well conserved among different orders of arthropods. University of Wisconsin Library 2008-09-23 /pmc/articles/PMC3062493/ /pubmed/20331401 http://dx.doi.org/10.1673/031.008.5401 Text en http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Yan, Liu Zhi-Qi, Meng Bao-Long, Niu Li-Hua, He Hong-Biao, Weng Wei-Feng, Shen Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori |
title | Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori
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title_full | Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori
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title_fullStr | Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori
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title_full_unstemmed | Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori
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title_short | Molecular Cloning and Analysis of the Tryptophan oxygenase Gene in the Silkworm, Bombyx mori
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title_sort | molecular cloning and analysis of the tryptophan oxygenase gene in the silkworm, bombyx mori |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062493/ https://www.ncbi.nlm.nih.gov/pubmed/20331401 http://dx.doi.org/10.1673/031.008.5401 |
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