Cargando…
Absence of Vsx1 expression in the normal and damaged mouse cornea
PURPOSE: To examine the expression of visual system homeobox 1 (Vsx1) in the mouse cornea and its potential role in the corneal wound response pathway. METHODS: Expression of Vsx1 was examined by quantitative reverse-transcription PCR (qRT–PCR) in corneal tissue from developing and adult mice and fr...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062518/ https://www.ncbi.nlm.nih.gov/pubmed/21437200 |
_version_ | 1782200710038814720 |
---|---|
author | Watson, Tom Chow, Robert L. |
author_facet | Watson, Tom Chow, Robert L. |
author_sort | Watson, Tom |
collection | PubMed |
description | PURPOSE: To examine the expression of visual system homeobox 1 (Vsx1) in the mouse cornea and its potential role in the corneal wound response pathway. METHODS: Expression of Vsx1 was examined by quantitative reverse-transcription PCR (qRT–PCR) in corneal tissue from developing and adult mice and from mice that had undergone alkali-burn corneal wounding. Immunolabeling and Vsx1 knock-in reporter gene expression in wild type and Vsx1 null-mice were used to confirm the qRT–PCR data. RESULTS: Using qRT–PCR, Vsx1 expression was not detected in either the postnatal or adult mouse cornea or in corneas following wounding. This qRT–PCR data was supported by the absence of specific Vsx1 immunolabeling and Vsx1 knock-in reporter expression in untreated and wounded corneas. CONCLUSIONS: In mice, Vsx1 mRNA, protein or reporter gene expression is not detected in the normal or damaged cornea. These results make it uncertain what role VSX1/Vsx1 plays in corneal biology. Future experiments examining the pathogenicity of VSX1 mutations associated with corneal dystrophy are required to rule out species differences and possible non-cell autonomous roles for VSX1 in the cornea. |
format | Text |
id | pubmed-3062518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-30625182011-03-23 Absence of Vsx1 expression in the normal and damaged mouse cornea Watson, Tom Chow, Robert L. Mol Vis Research Article PURPOSE: To examine the expression of visual system homeobox 1 (Vsx1) in the mouse cornea and its potential role in the corneal wound response pathway. METHODS: Expression of Vsx1 was examined by quantitative reverse-transcription PCR (qRT–PCR) in corneal tissue from developing and adult mice and from mice that had undergone alkali-burn corneal wounding. Immunolabeling and Vsx1 knock-in reporter gene expression in wild type and Vsx1 null-mice were used to confirm the qRT–PCR data. RESULTS: Using qRT–PCR, Vsx1 expression was not detected in either the postnatal or adult mouse cornea or in corneas following wounding. This qRT–PCR data was supported by the absence of specific Vsx1 immunolabeling and Vsx1 knock-in reporter expression in untreated and wounded corneas. CONCLUSIONS: In mice, Vsx1 mRNA, protein or reporter gene expression is not detected in the normal or damaged cornea. These results make it uncertain what role VSX1/Vsx1 plays in corneal biology. Future experiments examining the pathogenicity of VSX1 mutations associated with corneal dystrophy are required to rule out species differences and possible non-cell autonomous roles for VSX1 in the cornea. Molecular Vision 2011-03-16 /pmc/articles/PMC3062518/ /pubmed/21437200 Text en Copyright © 2011 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Watson, Tom Chow, Robert L. Absence of Vsx1 expression in the normal and damaged mouse cornea |
title | Absence of Vsx1 expression in the normal and damaged mouse cornea |
title_full | Absence of Vsx1 expression in the normal and damaged mouse cornea |
title_fullStr | Absence of Vsx1 expression in the normal and damaged mouse cornea |
title_full_unstemmed | Absence of Vsx1 expression in the normal and damaged mouse cornea |
title_short | Absence of Vsx1 expression in the normal and damaged mouse cornea |
title_sort | absence of vsx1 expression in the normal and damaged mouse cornea |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062518/ https://www.ncbi.nlm.nih.gov/pubmed/21437200 |
work_keys_str_mv | AT watsontom absenceofvsx1expressioninthenormalanddamagedmousecornea AT chowrobertl absenceofvsx1expressioninthenormalanddamagedmousecornea |