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The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling

The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that...

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Autores principales: Schmidt, Juliane, Müsken, Mathias, Becker, Tanja, Magnowska, Zofia, Bertinetti, Daniela, Möller, Stefan, Zimmermann, Bastian, Herberg, Friedrich W., Jänsch, Lothar, Häussler, Susanne
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062574/
https://www.ncbi.nlm.nih.gov/pubmed/21445368
http://dx.doi.org/10.1371/journal.pone.0018184
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author Schmidt, Juliane
Müsken, Mathias
Becker, Tanja
Magnowska, Zofia
Bertinetti, Daniela
Möller, Stefan
Zimmermann, Bastian
Herberg, Friedrich W.
Jänsch, Lothar
Häussler, Susanne
author_facet Schmidt, Juliane
Müsken, Mathias
Becker, Tanja
Magnowska, Zofia
Bertinetti, Daniela
Möller, Stefan
Zimmermann, Bastian
Herberg, Friedrich W.
Jänsch, Lothar
Häussler, Susanne
author_sort Schmidt, Juliane
collection PubMed
description The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa.
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spelling pubmed-30625742011-03-28 The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling Schmidt, Juliane Müsken, Mathias Becker, Tanja Magnowska, Zofia Bertinetti, Daniela Möller, Stefan Zimmermann, Bastian Herberg, Friedrich W. Jänsch, Lothar Häussler, Susanne PLoS One Research Article The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have demonstrated that motility, including swimming, swarming and twitching, seems to play an important role in the surface colonization and establishment of structured biofilms. Thereby, the impact of chemotaxis on biofilm formation has been less intensively studied. Pseudomonas aeruginosa has a very complex chemosensory system with two Che systems implicated in flagella-mediated motility. In this study, we demonstrate that the chemotaxis protein CheR1 is a methyltransferase that binds S-adenosylmethionine and transfers a methyl group from this methyl donor to the chemoreceptor PctA, an activity which can be stimulated by the attractant serine but not by glutamine. We furthermore demonstrate that CheR1 does not only play a role in flagella-mediated chemotaxis but that its activity is essential for the formation and maintenance of bacterial biofilm structures. We propose a model in which motility and chemotaxis impact on initial attachment processes, dispersion and reattachment and increase the efficiency and frequency of surface sampling in P. aeruginosa. Public Library of Science 2011-03-22 /pmc/articles/PMC3062574/ /pubmed/21445368 http://dx.doi.org/10.1371/journal.pone.0018184 Text en Schmidt et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Schmidt, Juliane
Müsken, Mathias
Becker, Tanja
Magnowska, Zofia
Bertinetti, Daniela
Möller, Stefan
Zimmermann, Bastian
Herberg, Friedrich W.
Jänsch, Lothar
Häussler, Susanne
The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling
title The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling
title_full The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling
title_fullStr The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling
title_full_unstemmed The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling
title_short The Pseudomonas aeruginosa Chemotaxis Methyltransferase CheR1 Impacts on Bacterial Surface Sampling
title_sort pseudomonas aeruginosa chemotaxis methyltransferase cher1 impacts on bacterial surface sampling
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062574/
https://www.ncbi.nlm.nih.gov/pubmed/21445368
http://dx.doi.org/10.1371/journal.pone.0018184
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