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Carbon monoxide prevents hepatic mitochondrial membrane permeabilization
BACKGROUND: Low concentrations of carbon monoxide (CO) protect hepatocytes against apoptosis and confers cytoprotection in several models of liver. Mitochondria are key organelles in cell death control via their membrane permeabilization and the release of pro-apoptotic factors. RESULTS: Herein, we...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062616/ https://www.ncbi.nlm.nih.gov/pubmed/21388535 http://dx.doi.org/10.1186/1471-2121-12-10 |
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author | Queiroga, Cláudia SF Almeida, Ana S Alves, Paula M Brenner, Catherine Vieira, Helena LA |
author_facet | Queiroga, Cláudia SF Almeida, Ana S Alves, Paula M Brenner, Catherine Vieira, Helena LA |
author_sort | Queiroga, Cláudia SF |
collection | PubMed |
description | BACKGROUND: Low concentrations of carbon monoxide (CO) protect hepatocytes against apoptosis and confers cytoprotection in several models of liver. Mitochondria are key organelles in cell death control via their membrane permeabilization and the release of pro-apoptotic factors. RESULTS: Herein, we show that CO prevents mitochondrial membrane permeabilization (MMP) in liver isolated mitochondria. Direct and indirect approaches were used to evaluate MMP inhibition by CO: mitochondrial swelling, mitochondrial depolarization and inner membrane permeabilization. Additionally, CO increases mitochondrial reactive oxygen species (ROS) generation, and their scavenging, by ß-carotene addition, decreases CO protection, which reveals the key role of ROS. Interestingly, cytochrome c oxidase transiently responds to low concentrations of CO by decreasing its activity in the first 5 min, later on there is an increase of cytochrome c oxidase activity, which were detected up to 30 min. CONCLUSION: CO directly prevents mitochondrial membrane permeabilization, which might be implicated in the hepatic apoptosis inhibition by this gaseoustransmitter. |
format | Text |
id | pubmed-3062616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30626162011-03-23 Carbon monoxide prevents hepatic mitochondrial membrane permeabilization Queiroga, Cláudia SF Almeida, Ana S Alves, Paula M Brenner, Catherine Vieira, Helena LA BMC Cell Biol Research Article BACKGROUND: Low concentrations of carbon monoxide (CO) protect hepatocytes against apoptosis and confers cytoprotection in several models of liver. Mitochondria are key organelles in cell death control via their membrane permeabilization and the release of pro-apoptotic factors. RESULTS: Herein, we show that CO prevents mitochondrial membrane permeabilization (MMP) in liver isolated mitochondria. Direct and indirect approaches were used to evaluate MMP inhibition by CO: mitochondrial swelling, mitochondrial depolarization and inner membrane permeabilization. Additionally, CO increases mitochondrial reactive oxygen species (ROS) generation, and their scavenging, by ß-carotene addition, decreases CO protection, which reveals the key role of ROS. Interestingly, cytochrome c oxidase transiently responds to low concentrations of CO by decreasing its activity in the first 5 min, later on there is an increase of cytochrome c oxidase activity, which were detected up to 30 min. CONCLUSION: CO directly prevents mitochondrial membrane permeabilization, which might be implicated in the hepatic apoptosis inhibition by this gaseoustransmitter. BioMed Central 2011-03-09 /pmc/articles/PMC3062616/ /pubmed/21388535 http://dx.doi.org/10.1186/1471-2121-12-10 Text en Copyright ©2011 Queiroga et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Queiroga, Cláudia SF Almeida, Ana S Alves, Paula M Brenner, Catherine Vieira, Helena LA Carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
title | Carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
title_full | Carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
title_fullStr | Carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
title_full_unstemmed | Carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
title_short | Carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
title_sort | carbon monoxide prevents hepatic mitochondrial membrane permeabilization |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062616/ https://www.ncbi.nlm.nih.gov/pubmed/21388535 http://dx.doi.org/10.1186/1471-2121-12-10 |
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