Cargando…

Gene expression throughout a vertebrate's embryogenesis

BACKGROUND: Describing the patterns of gene expression during embryonic development has broadened our understanding of the processes and patterns that define morphogenesis. Yet gene expression patterns have not been described throughout vertebrate embryogenesis. This study presents statistical analy...

Descripción completa

Detalles Bibliográficos
Autores principales: Bozinovic, Goran, Sit, Tim L, Hinton, David E, Oleksiak, Marjorie F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062618/
https://www.ncbi.nlm.nih.gov/pubmed/21356103
http://dx.doi.org/10.1186/1471-2164-12-132
_version_ 1782200731489533952
author Bozinovic, Goran
Sit, Tim L
Hinton, David E
Oleksiak, Marjorie F
author_facet Bozinovic, Goran
Sit, Tim L
Hinton, David E
Oleksiak, Marjorie F
author_sort Bozinovic, Goran
collection PubMed
description BACKGROUND: Describing the patterns of gene expression during embryonic development has broadened our understanding of the processes and patterns that define morphogenesis. Yet gene expression patterns have not been described throughout vertebrate embryogenesis. This study presents statistical analyses of gene expression during all 40 developmental stages in the teleost Fundulus heteroclitus using four biological replicates per stage. RESULTS: Patterns of gene expression for 7,000 genes appear to be important as they recapitulate developmental timing. Among the 45% of genes with significant expression differences between pairs of temporally adjacent stages, significant differences in gene expression vary from as few as five to more than 660. Five adjacent stages have disproportionately more significant changes in gene expression (> 200 genes) relative to other stages: four to eight and eight to sixteen cell stages, onset of circulation, pre and post-hatch, and during complete yolk absorption. The fewest differences among adjacent stages occur during gastrulation. Yet, at stage 16, (pre-mid-gastrulation) the largest number of genes has peak expression. This stage has an over representation of genes in oxidative respiration and protein expression (ribosomes, translational genes and proteases). Unexpectedly, among all ribosomal genes, both strong positive and negative correlations occur. Similar correlated patterns of expression occur among all significant genes. CONCLUSIONS: These data provide statistical support for the temporal dynamics of developmental gene expression during all stages of vertebrate development.
format Text
id pubmed-3062618
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30626182011-03-23 Gene expression throughout a vertebrate's embryogenesis Bozinovic, Goran Sit, Tim L Hinton, David E Oleksiak, Marjorie F BMC Genomics Research Article BACKGROUND: Describing the patterns of gene expression during embryonic development has broadened our understanding of the processes and patterns that define morphogenesis. Yet gene expression patterns have not been described throughout vertebrate embryogenesis. This study presents statistical analyses of gene expression during all 40 developmental stages in the teleost Fundulus heteroclitus using four biological replicates per stage. RESULTS: Patterns of gene expression for 7,000 genes appear to be important as they recapitulate developmental timing. Among the 45% of genes with significant expression differences between pairs of temporally adjacent stages, significant differences in gene expression vary from as few as five to more than 660. Five adjacent stages have disproportionately more significant changes in gene expression (> 200 genes) relative to other stages: four to eight and eight to sixteen cell stages, onset of circulation, pre and post-hatch, and during complete yolk absorption. The fewest differences among adjacent stages occur during gastrulation. Yet, at stage 16, (pre-mid-gastrulation) the largest number of genes has peak expression. This stage has an over representation of genes in oxidative respiration and protein expression (ribosomes, translational genes and proteases). Unexpectedly, among all ribosomal genes, both strong positive and negative correlations occur. Similar correlated patterns of expression occur among all significant genes. CONCLUSIONS: These data provide statistical support for the temporal dynamics of developmental gene expression during all stages of vertebrate development. BioMed Central 2011-02-28 /pmc/articles/PMC3062618/ /pubmed/21356103 http://dx.doi.org/10.1186/1471-2164-12-132 Text en Copyright ©2011 Bozinovic et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bozinovic, Goran
Sit, Tim L
Hinton, David E
Oleksiak, Marjorie F
Gene expression throughout a vertebrate's embryogenesis
title Gene expression throughout a vertebrate's embryogenesis
title_full Gene expression throughout a vertebrate's embryogenesis
title_fullStr Gene expression throughout a vertebrate's embryogenesis
title_full_unstemmed Gene expression throughout a vertebrate's embryogenesis
title_short Gene expression throughout a vertebrate's embryogenesis
title_sort gene expression throughout a vertebrate's embryogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062618/
https://www.ncbi.nlm.nih.gov/pubmed/21356103
http://dx.doi.org/10.1186/1471-2164-12-132
work_keys_str_mv AT bozinovicgoran geneexpressionthroughoutavertebratesembryogenesis
AT sittiml geneexpressionthroughoutavertebratesembryogenesis
AT hintondavide geneexpressionthroughoutavertebratesembryogenesis
AT oleksiakmarjorief geneexpressionthroughoutavertebratesembryogenesis