The preparation of an infectious full-length cDNA clone of Saffold virus

The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrat...

Descripción completa

Detalles Bibliográficos
Autores principales: Himeda, Toshiki, Hosomi, Takushi, Asif, Naeem, Shimizu, Hiroyuki, Okuwa, Takako, Muraki, Yasushi, Ohara, Yoshiro
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062622/
https://www.ncbi.nlm.nih.gov/pubmed/21385468
http://dx.doi.org/10.1186/1743-422X-8-110
_version_ 1782200732440592384
author Himeda, Toshiki
Hosomi, Takushi
Asif, Naeem
Shimizu, Hiroyuki
Okuwa, Takako
Muraki, Yasushi
Ohara, Yoshiro
author_facet Himeda, Toshiki
Hosomi, Takushi
Asif, Naeem
Shimizu, Hiroyuki
Okuwa, Takako
Muraki, Yasushi
Ohara, Yoshiro
author_sort Himeda, Toshiki
collection PubMed
description The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV.
format Text
id pubmed-3062622
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30626222011-03-23 The preparation of an infectious full-length cDNA clone of Saffold virus Himeda, Toshiki Hosomi, Takushi Asif, Naeem Shimizu, Hiroyuki Okuwa, Takako Muraki, Yasushi Ohara, Yoshiro Virol J Methodology The pathogenicity of Saffold virus (SAFV) among humans still remains unclear, although it was identified as a novel human cardiovirus in 2007. In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. To obtain the infectious RNA using T7 promoter, a variant of T7 RNA polymerase, which fails to recognize the PTH signal, was useful. This study will provide a valuable technical insight into the reverse genetics of SAFV. BioMed Central 2011-03-09 /pmc/articles/PMC3062622/ /pubmed/21385468 http://dx.doi.org/10.1186/1743-422X-8-110 Text en Copyright ©2011 Himeda et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Himeda, Toshiki
Hosomi, Takushi
Asif, Naeem
Shimizu, Hiroyuki
Okuwa, Takako
Muraki, Yasushi
Ohara, Yoshiro
The preparation of an infectious full-length cDNA clone of Saffold virus
title The preparation of an infectious full-length cDNA clone of Saffold virus
title_full The preparation of an infectious full-length cDNA clone of Saffold virus
title_fullStr The preparation of an infectious full-length cDNA clone of Saffold virus
title_full_unstemmed The preparation of an infectious full-length cDNA clone of Saffold virus
title_short The preparation of an infectious full-length cDNA clone of Saffold virus
title_sort preparation of an infectious full-length cdna clone of saffold virus
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062622/
https://www.ncbi.nlm.nih.gov/pubmed/21385468
http://dx.doi.org/10.1186/1743-422X-8-110
work_keys_str_mv AT himedatoshiki thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT hosomitakushi thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT asifnaeem thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT shimizuhiroyuki thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT okuwatakako thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT murakiyasushi thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT oharayoshiro thepreparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT himedatoshiki preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT hosomitakushi preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT asifnaeem preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT shimizuhiroyuki preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT okuwatakako preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT murakiyasushi preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus
AT oharayoshiro preparationofaninfectiousfulllengthcdnacloneofsaffoldvirus

Ejemplares similares