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Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin

In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isola...

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Autores principales: Sanarico, Nunzia, Colone, Alessia, Grassi, Manuela, Speranza, Viviana, Giovannini, Daniela, Ciaramella, Antonio, Colizzi, Vittorio, Mariani, Francesca
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062957/
https://www.ncbi.nlm.nih.gov/pubmed/21436989
http://dx.doi.org/10.1155/2011/741051
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author Sanarico, Nunzia
Colone, Alessia
Grassi, Manuela
Speranza, Viviana
Giovannini, Daniela
Ciaramella, Antonio
Colizzi, Vittorio
Mariani, Francesca
author_facet Sanarico, Nunzia
Colone, Alessia
Grassi, Manuela
Speranza, Viviana
Giovannini, Daniela
Ciaramella, Antonio
Colizzi, Vittorio
Mariani, Francesca
author_sort Sanarico, Nunzia
collection PubMed
description In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response.
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spelling pubmed-30629572011-03-24 Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin Sanarico, Nunzia Colone, Alessia Grassi, Manuela Speranza, Viviana Giovannini, Daniela Ciaramella, Antonio Colizzi, Vittorio Mariani, Francesca Clin Dev Immunol Research Article In order to analyze dendritic cells (DCs) activation following infection with different mycobacterial strains, we studied the expression profiles of 165 genes of human monocyte-derived DCs infected with H37Rv, a virulent Mycobacterium tuberculosis (MTB) laboratory strain, CMT97, a clinical MTB isolate, Mycobacterium bovis bacillus Calmette-Guérin (BCG), Aventis Pasteur, and BCG Japan, both employed as vaccine against tuberculosis. The analysis of the gene expression reveals that, despite a set of genes similarly modulated, DCs response resulted strain dependent. In particular, H37Rv significantly upregulated EBI3 expression compared with BCG Japan, while it was the only strain that failed to release a significant IL-10 amount. Of note, BCG Japan showed a marked increase in CCR7 and TNF-α expression regarding both MTB strains and it resulted the only strain failing in exponential intracellular growth. Our results suggest that DCs display the ability to elicit a tailored strain-specific immune response. Hindawi Publishing Corporation 2011 2011-03-22 /pmc/articles/PMC3062957/ /pubmed/21436989 http://dx.doi.org/10.1155/2011/741051 Text en Copyright © 2011 Nunzia Sanarico et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sanarico, Nunzia
Colone, Alessia
Grassi, Manuela
Speranza, Viviana
Giovannini, Daniela
Ciaramella, Antonio
Colizzi, Vittorio
Mariani, Francesca
Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
title Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
title_full Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
title_fullStr Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
title_full_unstemmed Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
title_short Different Transcriptional Profiles of Human Monocyte-Derived Dendritic Cells Infected with Distinct Strains of Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin
title_sort different transcriptional profiles of human monocyte-derived dendritic cells infected with distinct strains of mycobacterium tuberculosis and mycobacterium bovis bacillus calmette-guérin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3062957/
https://www.ncbi.nlm.nih.gov/pubmed/21436989
http://dx.doi.org/10.1155/2011/741051
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