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RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima

BACKGROUND: Cytomegalovirus (CMV) infection has been associated with accelerated transplant vasculopathy. In this study, we assessed the effects of acute rat CMV (RCMV) infection on vessel remodeling in transplant vasculopathy, focusing on allograft morphology, inflammation and contribution of adven...

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Autores principales: Grudzinska, Monika K, Bojakowski, Krzysztof, Soin, Joanna, Stassen, Frank, Söderberg-Nauclér, Cecilia, Religa, Piotr
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063229/
https://www.ncbi.nlm.nih.gov/pubmed/21429242
http://dx.doi.org/10.1186/2042-4280-1-7
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author Grudzinska, Monika K
Bojakowski, Krzysztof
Soin, Joanna
Stassen, Frank
Söderberg-Nauclér, Cecilia
Religa, Piotr
author_facet Grudzinska, Monika K
Bojakowski, Krzysztof
Soin, Joanna
Stassen, Frank
Söderberg-Nauclér, Cecilia
Religa, Piotr
author_sort Grudzinska, Monika K
collection PubMed
description BACKGROUND: Cytomegalovirus (CMV) infection has been associated with accelerated transplant vasculopathy. In this study, we assessed the effects of acute rat CMV (RCMV) infection on vessel remodeling in transplant vasculopathy, focusing on allograft morphology, inflammation and contribution of adventitial cells to intimal hyperplasia. METHODS: Infrarenal aorta was locally infected with RCMV and transplanted from female F344 rats to male Lewis rats. Graft samples were collected 2 and 8 weeks after transplantation and analyzed for intimal hyperplasia, collagen degradation and inflammation. Transplantation of aorta followed by transplantation of RCMV infected and labeled isogenic adventitia were performed to study migration of adventitial cells towards the intima. RESULTS: Intimal hyperplasia was increased threefold in infected allografts. RCMV induced apoptosis in the media, expression of matrix metalloproteinase 2, and decreased collagen deposits. Macrophage infiltration was increased in the infected allografts and resulted in increased production of MCP-1. RCMV-infected macrophages were observed in the adventitia and intima. Cells derived from infected adventitia migrated towards the intima of the allograft. CONCLUSIONS: RCMV enhances infiltration of macrophages to the allografts, and thereby increases MCP-1 production and inflammation, followed by recruitment of adventitial cells to the intima and accelerated intimal hyperplasia.
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spelling pubmed-30632292011-03-24 RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima Grudzinska, Monika K Bojakowski, Krzysztof Soin, Joanna Stassen, Frank Söderberg-Nauclér, Cecilia Religa, Piotr Herpesviridae Research BACKGROUND: Cytomegalovirus (CMV) infection has been associated with accelerated transplant vasculopathy. In this study, we assessed the effects of acute rat CMV (RCMV) infection on vessel remodeling in transplant vasculopathy, focusing on allograft morphology, inflammation and contribution of adventitial cells to intimal hyperplasia. METHODS: Infrarenal aorta was locally infected with RCMV and transplanted from female F344 rats to male Lewis rats. Graft samples were collected 2 and 8 weeks after transplantation and analyzed for intimal hyperplasia, collagen degradation and inflammation. Transplantation of aorta followed by transplantation of RCMV infected and labeled isogenic adventitia were performed to study migration of adventitial cells towards the intima. RESULTS: Intimal hyperplasia was increased threefold in infected allografts. RCMV induced apoptosis in the media, expression of matrix metalloproteinase 2, and decreased collagen deposits. Macrophage infiltration was increased in the infected allografts and resulted in increased production of MCP-1. RCMV-infected macrophages were observed in the adventitia and intima. Cells derived from infected adventitia migrated towards the intima of the allograft. CONCLUSIONS: RCMV enhances infiltration of macrophages to the allografts, and thereby increases MCP-1 production and inflammation, followed by recruitment of adventitial cells to the intima and accelerated intimal hyperplasia. BioMed Central 2010-12-23 /pmc/articles/PMC3063229/ /pubmed/21429242 http://dx.doi.org/10.1186/2042-4280-1-7 Text en Copyright ©2010 Grudzinska et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Grudzinska, Monika K
Bojakowski, Krzysztof
Soin, Joanna
Stassen, Frank
Söderberg-Nauclér, Cecilia
Religa, Piotr
RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima
title RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima
title_full RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima
title_fullStr RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima
title_full_unstemmed RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima
title_short RCMV increases intimal hyperplasia by inducing inflammation, MCP-1 expression and recruitment of adventitial cells to intima
title_sort rcmv increases intimal hyperplasia by inducing inflammation, mcp-1 expression and recruitment of adventitial cells to intima
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063229/
https://www.ncbi.nlm.nih.gov/pubmed/21429242
http://dx.doi.org/10.1186/2042-4280-1-7
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