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Substrate-dependent control of MAPK phosphorylation in vivo
Phosphorylation of the mitogen-activated protein kinase (MAPK) is essential for its enzymatic activity and ability to control multiple substrates inside a cell. According to the current models, control of MAPK phosphorylation is independent of its substrates, which are viewed as mere sensors of MAPK...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063690/ https://www.ncbi.nlm.nih.gov/pubmed/21283143 http://dx.doi.org/10.1038/msb.2010.121 |
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author | Kim, Yoosik Paroush, Ze'ev Nairz, Knud Hafen, Ernst Jiménez, Gerardo Shvartsman, Stanislav Y |
author_facet | Kim, Yoosik Paroush, Ze'ev Nairz, Knud Hafen, Ernst Jiménez, Gerardo Shvartsman, Stanislav Y |
author_sort | Kim, Yoosik |
collection | PubMed |
description | Phosphorylation of the mitogen-activated protein kinase (MAPK) is essential for its enzymatic activity and ability to control multiple substrates inside a cell. According to the current models, control of MAPK phosphorylation is independent of its substrates, which are viewed as mere sensors of MAPK activity. Contrary to this modular view of MAPK signaling, our studies in the Drosophila embryo demonstrate that substrates can regulate the level of MAPK phosphorylation in vivo. We demonstrate that a twofold change in the gene dosage of a single substrate can induce a significant change in the phosphorylation level of MAPK and in the conversion of other substrates. Our results support a model where substrates of MAPK counteract its dephosphorylation by phosphatases. Substrate-dependent control of MAPK phosphorylation is a manifestation of a more general retroactive effect that should be intrinsic to all networks with covalent modification cycles. |
format | Text |
id | pubmed-3063690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-30636902011-03-25 Substrate-dependent control of MAPK phosphorylation in vivo Kim, Yoosik Paroush, Ze'ev Nairz, Knud Hafen, Ernst Jiménez, Gerardo Shvartsman, Stanislav Y Mol Syst Biol Report Phosphorylation of the mitogen-activated protein kinase (MAPK) is essential for its enzymatic activity and ability to control multiple substrates inside a cell. According to the current models, control of MAPK phosphorylation is independent of its substrates, which are viewed as mere sensors of MAPK activity. Contrary to this modular view of MAPK signaling, our studies in the Drosophila embryo demonstrate that substrates can regulate the level of MAPK phosphorylation in vivo. We demonstrate that a twofold change in the gene dosage of a single substrate can induce a significant change in the phosphorylation level of MAPK and in the conversion of other substrates. Our results support a model where substrates of MAPK counteract its dephosphorylation by phosphatases. Substrate-dependent control of MAPK phosphorylation is a manifestation of a more general retroactive effect that should be intrinsic to all networks with covalent modification cycles. European Molecular Biology Organization 2011-02-01 /pmc/articles/PMC3063690/ /pubmed/21283143 http://dx.doi.org/10.1038/msb.2010.121 Text en Copyright © 2011, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-nd/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Report Kim, Yoosik Paroush, Ze'ev Nairz, Knud Hafen, Ernst Jiménez, Gerardo Shvartsman, Stanislav Y Substrate-dependent control of MAPK phosphorylation in vivo |
title | Substrate-dependent control of MAPK phosphorylation in vivo |
title_full | Substrate-dependent control of MAPK phosphorylation in vivo |
title_fullStr | Substrate-dependent control of MAPK phosphorylation in vivo |
title_full_unstemmed | Substrate-dependent control of MAPK phosphorylation in vivo |
title_short | Substrate-dependent control of MAPK phosphorylation in vivo |
title_sort | substrate-dependent control of mapk phosphorylation in vivo |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063690/ https://www.ncbi.nlm.nih.gov/pubmed/21283143 http://dx.doi.org/10.1038/msb.2010.121 |
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