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Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo

Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated...

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Autores principales: Waite, Janelle C., Leiner, Ingrid, Lauer, Peter, Rae, Chris S., Barbet, Gaetan, Zheng, Huan, Portnoy, Daniel A., Pamer, Eric G., Dustin, Michael L.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063765/
https://www.ncbi.nlm.nih.gov/pubmed/21455492
http://dx.doi.org/10.1371/journal.ppat.1001326
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author Waite, Janelle C.
Leiner, Ingrid
Lauer, Peter
Rae, Chris S.
Barbet, Gaetan
Zheng, Huan
Portnoy, Daniel A.
Pamer, Eric G.
Dustin, Michael L.
author_facet Waite, Janelle C.
Leiner, Ingrid
Lauer, Peter
Rae, Chris S.
Barbet, Gaetan
Zheng, Huan
Portnoy, Daniel A.
Pamer, Eric G.
Dustin, Michael L.
author_sort Waite, Janelle C.
collection PubMed
description Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8(+) T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8(+) T cells in effector sites.
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spelling pubmed-30637652011-03-31 Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. PLoS Pathog Research Article Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8(+) T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8(+) T cells in effector sites. Public Library of Science 2011-03-24 /pmc/articles/PMC3063765/ /pubmed/21455492 http://dx.doi.org/10.1371/journal.ppat.1001326 Text en Waite et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Waite, Janelle C.
Leiner, Ingrid
Lauer, Peter
Rae, Chris S.
Barbet, Gaetan
Zheng, Huan
Portnoy, Daniel A.
Pamer, Eric G.
Dustin, Michael L.
Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
title Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
title_full Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
title_fullStr Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
title_full_unstemmed Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
title_short Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
title_sort dynamic imaging of the effector immune response to listeria infection in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063765/
https://www.ncbi.nlm.nih.gov/pubmed/21455492
http://dx.doi.org/10.1371/journal.ppat.1001326
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