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Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063765/ https://www.ncbi.nlm.nih.gov/pubmed/21455492 http://dx.doi.org/10.1371/journal.ppat.1001326 |
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author | Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. |
author_facet | Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. |
author_sort | Waite, Janelle C. |
collection | PubMed |
description | Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8(+) T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8(+) T cells in effector sites. |
format | Text |
id | pubmed-3063765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30637652011-03-31 Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. PLoS Pathog Research Article Host defense against the intracellular pathogen Listeria monocytogenes (Lm) requires innate and adaptive immunity. Here, we directly imaged immune cell dynamics at Lm foci established by dendritic cells in the subcapsular red pulp (scDC) using intravital microscopy. Blood borne Lm rapidly associated with scDC. Myelomonocytic cells (MMC) swarmed around non-motile scDC forming foci from which blood flow was excluded. The depletion of scDC after foci were established resulted in a 10-fold reduction in viable Lm, while graded depletion of MMC resulted in 30–1000 fold increase in viable Lm in foci with enhanced blood flow. Effector CD8(+) T cells at sites of infection displayed a two-tiered reduction in motility with antigen independent and antigen dependent components, including stable interactions with infected and non-infected scDC. Thus, swarming MMC contribute to control of Lm prior to development of T cell immunity by direct killing and sequestration from blood flow, while scDC appear to promote Lm survival while preferentially interacting with CD8(+) T cells in effector sites. Public Library of Science 2011-03-24 /pmc/articles/PMC3063765/ /pubmed/21455492 http://dx.doi.org/10.1371/journal.ppat.1001326 Text en Waite et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Waite, Janelle C. Leiner, Ingrid Lauer, Peter Rae, Chris S. Barbet, Gaetan Zheng, Huan Portnoy, Daniel A. Pamer, Eric G. Dustin, Michael L. Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo |
title | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
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title_full | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
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title_fullStr | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
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title_full_unstemmed | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
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title_short | Dynamic Imaging of the Effector Immune Response to Listeria Infection In Vivo
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title_sort | dynamic imaging of the effector immune response to listeria infection in vivo |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063765/ https://www.ncbi.nlm.nih.gov/pubmed/21455492 http://dx.doi.org/10.1371/journal.ppat.1001326 |
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