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Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008
BACKGROUND: The dominant viral etiologies responsible for acute respiratory infections (ARIs) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by ARIs are highest. Improved etiological insight is needed to imp...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063798/ https://www.ncbi.nlm.nih.gov/pubmed/21455313 http://dx.doi.org/10.1371/journal.pone.0018176 |
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author | Do, Anh Ha Lien van Doorn, H. Rogier Nghiem, My Ngoc Bryant, Juliet E. Hoang, Thanh Hang thi Do, Quang Ha Le Van, Tan Tran, Tan Thanh Wills, Bridget van Nguyen, Vinh Chau Vo, Minh Hien Vo, Cong Khanh Nguyen, Minh Dung Farrar, Jeremy Tran, Tinh Hien de Jong, Menno D. |
author_facet | Do, Anh Ha Lien van Doorn, H. Rogier Nghiem, My Ngoc Bryant, Juliet E. Hoang, Thanh Hang thi Do, Quang Ha Le Van, Tan Tran, Tan Thanh Wills, Bridget van Nguyen, Vinh Chau Vo, Minh Hien Vo, Cong Khanh Nguyen, Minh Dung Farrar, Jeremy Tran, Tinh Hien de Jong, Menno D. |
author_sort | Do, Anh Ha Lien |
collection | PubMed |
description | BACKGROUND: The dominant viral etiologies responsible for acute respiratory infections (ARIs) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by ARIs are highest. Improved etiological insight is needed to improve clinical management and prevention. OBJECTIVES: We conducted a three-year prospective descriptive study of severe respiratory illness among children from 2 months to 13 years of age within the largest referral hospital for infectious diseases in southern Vietnam. METHODS: Molecular detection for 15 viral species and subtypes was performed on three types of respiratory specimens (nose, throat swabs and nasopharyngeal aspirates) using a multiplex RT-PCR kit (Seeplex™ RV detection, Seegene) and additional monoplex real-time RT-PCRs. RESULTS: A total of 309 children were enrolled from November 2004 to January 2008. Viruses were identified in 72% (222/309) of cases, including respiratory syncytial virus (24%), influenza virus A and B (17%), human bocavirus (16%), enterovirus (9%), human coronavirus (8%), human metapneumovirus (7%), parainfluenza virus 1–3 (6%), adenovirus (5%), and human rhinovirus A (4%). Co-infections with multiple viruses were detected in 20% (62/309) of patients. When combined, diagnostic yields in nose and throat swabs were similar to nasopharyngeal aspirates. CONCLUSION: Similar to other parts in the world, RSV and influenza were the predominant viral pathogens detected in Vietnamese hospitalized children. Combined nasal and throat swabs are the specimens of choice for sensitive molecular detection of a broad panel of viral agents. Further research is required to better understand the clinical significance of single versus multiple viral coinfections and to address the role of bacterial (co-)infections involved in severe respiratory illness. |
format | Text |
id | pubmed-3063798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30637982011-03-31 Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 Do, Anh Ha Lien van Doorn, H. Rogier Nghiem, My Ngoc Bryant, Juliet E. Hoang, Thanh Hang thi Do, Quang Ha Le Van, Tan Tran, Tan Thanh Wills, Bridget van Nguyen, Vinh Chau Vo, Minh Hien Vo, Cong Khanh Nguyen, Minh Dung Farrar, Jeremy Tran, Tinh Hien de Jong, Menno D. PLoS One Research Article BACKGROUND: The dominant viral etiologies responsible for acute respiratory infections (ARIs) are poorly understood, particularly among hospitalized children in resource-limited tropical countries where morbidity and mortality caused by ARIs are highest. Improved etiological insight is needed to improve clinical management and prevention. OBJECTIVES: We conducted a three-year prospective descriptive study of severe respiratory illness among children from 2 months to 13 years of age within the largest referral hospital for infectious diseases in southern Vietnam. METHODS: Molecular detection for 15 viral species and subtypes was performed on three types of respiratory specimens (nose, throat swabs and nasopharyngeal aspirates) using a multiplex RT-PCR kit (Seeplex™ RV detection, Seegene) and additional monoplex real-time RT-PCRs. RESULTS: A total of 309 children were enrolled from November 2004 to January 2008. Viruses were identified in 72% (222/309) of cases, including respiratory syncytial virus (24%), influenza virus A and B (17%), human bocavirus (16%), enterovirus (9%), human coronavirus (8%), human metapneumovirus (7%), parainfluenza virus 1–3 (6%), adenovirus (5%), and human rhinovirus A (4%). Co-infections with multiple viruses were detected in 20% (62/309) of patients. When combined, diagnostic yields in nose and throat swabs were similar to nasopharyngeal aspirates. CONCLUSION: Similar to other parts in the world, RSV and influenza were the predominant viral pathogens detected in Vietnamese hospitalized children. Combined nasal and throat swabs are the specimens of choice for sensitive molecular detection of a broad panel of viral agents. Further research is required to better understand the clinical significance of single versus multiple viral coinfections and to address the role of bacterial (co-)infections involved in severe respiratory illness. Public Library of Science 2011-03-24 /pmc/articles/PMC3063798/ /pubmed/21455313 http://dx.doi.org/10.1371/journal.pone.0018176 Text en Do et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Do, Anh Ha Lien van Doorn, H. Rogier Nghiem, My Ngoc Bryant, Juliet E. Hoang, Thanh Hang thi Do, Quang Ha Le Van, Tan Tran, Tan Thanh Wills, Bridget van Nguyen, Vinh Chau Vo, Minh Hien Vo, Cong Khanh Nguyen, Minh Dung Farrar, Jeremy Tran, Tinh Hien de Jong, Menno D. Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 |
title | Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 |
title_full | Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 |
title_fullStr | Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 |
title_full_unstemmed | Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 |
title_short | Viral Etiologies of Acute Respiratory Infections among Hospitalized Vietnamese Children in Ho Chi Minh City, 2004–2008 |
title_sort | viral etiologies of acute respiratory infections among hospitalized vietnamese children in ho chi minh city, 2004–2008 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3063798/ https://www.ncbi.nlm.nih.gov/pubmed/21455313 http://dx.doi.org/10.1371/journal.pone.0018176 |
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