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Modulation of Human Immune Responses by Bovine Interleukin-10

Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood mon...

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Autores principales: den Hartog, Gerco, Savelkoul, Huub F. J., Schoemaker, Ruud, Tijhaar, Edwin, Westphal, Adrie H., de Ruiter, Talitha, van de Weg-Schrijver, Elise, van Neerven, R. J. Joost
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064667/
https://www.ncbi.nlm.nih.gov/pubmed/21464967
http://dx.doi.org/10.1371/journal.pone.0018188
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author den Hartog, Gerco
Savelkoul, Huub F. J.
Schoemaker, Ruud
Tijhaar, Edwin
Westphal, Adrie H.
de Ruiter, Talitha
van de Weg-Schrijver, Elise
van Neerven, R. J. Joost
author_facet den Hartog, Gerco
Savelkoul, Huub F. J.
Schoemaker, Ruud
Tijhaar, Edwin
Westphal, Adrie H.
de Ruiter, Talitha
van de Weg-Schrijver, Elise
van Neerven, R. J. Joost
author_sort den Hartog, Gerco
collection PubMed
description Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets.
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spelling pubmed-30646672011-04-04 Modulation of Human Immune Responses by Bovine Interleukin-10 den Hartog, Gerco Savelkoul, Huub F. J. Schoemaker, Ruud Tijhaar, Edwin Westphal, Adrie H. de Ruiter, Talitha van de Weg-Schrijver, Elise van Neerven, R. J. Joost PLoS One Research Article Cytokines can be functionally active across species barriers. Bovine IL-10 has an amino acid sequence identity with human IL-10 of 76.8%. Therefore, the aim of this study was to evaluate whether bovine IL-10 has immunomodulatory activities on human monocytes and dendritic cells. Peripheral blood monocytes were isolated from healthy donors, and used directly or allowed to differentiate to dendritic cells under the influence of IL-4 and GM-CSF. Recombinant bovine IL-10 inhibited TLR induced activation of monocytes, and dose-dependently inhibited LPS-induced activation of monocyte-derived DCs comparable to human IL-10. By using blocking antibodies to either bovine IL-10 or the human IL-10 receptor it was demonstrated that inhibition of monocyte activation by bovine IL-10 was dependent on binding of bovine IL-10 to the human IL-10R. These data demonstrate that bovine IL-10 potently inhibits the activation of human myeloid cells in response to TLR activation. Bovine IL-10 present in dairy products may thus potentially contribute to the prevention of necrotizing enterocolitis and allergy, enhance mucosal tolerance induction and decrease intestinal inflammation and may therefore be applicable in infant foods and in immunomodulatory diets. Public Library of Science 2011-03-25 /pmc/articles/PMC3064667/ /pubmed/21464967 http://dx.doi.org/10.1371/journal.pone.0018188 Text en den Hartog et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
den Hartog, Gerco
Savelkoul, Huub F. J.
Schoemaker, Ruud
Tijhaar, Edwin
Westphal, Adrie H.
de Ruiter, Talitha
van de Weg-Schrijver, Elise
van Neerven, R. J. Joost
Modulation of Human Immune Responses by Bovine Interleukin-10
title Modulation of Human Immune Responses by Bovine Interleukin-10
title_full Modulation of Human Immune Responses by Bovine Interleukin-10
title_fullStr Modulation of Human Immune Responses by Bovine Interleukin-10
title_full_unstemmed Modulation of Human Immune Responses by Bovine Interleukin-10
title_short Modulation of Human Immune Responses by Bovine Interleukin-10
title_sort modulation of human immune responses by bovine interleukin-10
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3064667/
https://www.ncbi.nlm.nih.gov/pubmed/21464967
http://dx.doi.org/10.1371/journal.pone.0018188
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