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XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function
Hox transcription factors provide positional information during patterning of the anteroposterior axis. Hox transcription factors can co-operatively bind with PBC-class co-factors, enhancing specificity and affinity for their appropriate binding sites. The nuclear localisation of these co-factors is...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065463/ https://www.ncbi.nlm.nih.gov/pubmed/21464931 http://dx.doi.org/10.1371/journal.pone.0018010 |
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author | In der Rieden, Paul M. J. Jansen, Hans J. Durston, Antony J. |
author_facet | In der Rieden, Paul M. J. Jansen, Hans J. Durston, Antony J. |
author_sort | In der Rieden, Paul M. J. |
collection | PubMed |
description | Hox transcription factors provide positional information during patterning of the anteroposterior axis. Hox transcription factors can co-operatively bind with PBC-class co-factors, enhancing specificity and affinity for their appropriate binding sites. The nuclear localisation of these co-factors is regulated by the Meis-class of homeodomain proteins. During development of the zebrafish hindbrain, Meis3 has previously been shown to synergise with Hoxb1 in the autoregulation of Hoxb1. In Xenopus XMeis3 posteriorises the embryo upon ectopic expression. Recently, an early temporally collinear expression sequence of Hox genes was detected in Xenopus gastrula mesoderm (see intro. P3). There is evidence that this sequence sets up the embryo's later axial Hox expression pattern by time-space translation. We investigated whether XMeis3 is involved in regulation of this early mesodermal Hox gene expression. Here, we present evidence that XMeis3 is necessary for expression of Hoxd1, Hoxb4 and Hoxc6 in mesoderm during gastrulation. In addition, we show that XMeis3 function is necessary for the progression of gastrulation. Finally, we present evidence for synergy between XMeis3 and Hoxd1 in Hoxd1 autoregulation in mesoderm during gastrulation. |
format | Text |
id | pubmed-3065463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30654632011-04-04 XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function In der Rieden, Paul M. J. Jansen, Hans J. Durston, Antony J. PLoS One Research Article Hox transcription factors provide positional information during patterning of the anteroposterior axis. Hox transcription factors can co-operatively bind with PBC-class co-factors, enhancing specificity and affinity for their appropriate binding sites. The nuclear localisation of these co-factors is regulated by the Meis-class of homeodomain proteins. During development of the zebrafish hindbrain, Meis3 has previously been shown to synergise with Hoxb1 in the autoregulation of Hoxb1. In Xenopus XMeis3 posteriorises the embryo upon ectopic expression. Recently, an early temporally collinear expression sequence of Hox genes was detected in Xenopus gastrula mesoderm (see intro. P3). There is evidence that this sequence sets up the embryo's later axial Hox expression pattern by time-space translation. We investigated whether XMeis3 is involved in regulation of this early mesodermal Hox gene expression. Here, we present evidence that XMeis3 is necessary for expression of Hoxd1, Hoxb4 and Hoxc6 in mesoderm during gastrulation. In addition, we show that XMeis3 function is necessary for the progression of gastrulation. Finally, we present evidence for synergy between XMeis3 and Hoxd1 in Hoxd1 autoregulation in mesoderm during gastrulation. Public Library of Science 2011-03-28 /pmc/articles/PMC3065463/ /pubmed/21464931 http://dx.doi.org/10.1371/journal.pone.0018010 Text en Durston et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article In der Rieden, Paul M. J. Jansen, Hans J. Durston, Antony J. XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function |
title |
XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function |
title_full |
XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function |
title_fullStr |
XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function |
title_full_unstemmed |
XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function |
title_short |
XMeis3 Is Necessary for Mesodermal Hox Gene Expression and Function |
title_sort | xmeis3 is necessary for mesodermal hox gene expression and function |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065463/ https://www.ncbi.nlm.nih.gov/pubmed/21464931 http://dx.doi.org/10.1371/journal.pone.0018010 |
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