LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release

BACKGROUND: Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us...

Descripción completa

Detalles Bibliográficos
Autores principales: Lundequist, Anders, Boyce, Joshua A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065470/
https://www.ncbi.nlm.nih.gov/pubmed/21464938
http://dx.doi.org/10.1371/journal.pone.0018192
_version_ 1782200989080616960
author Lundequist, Anders
Boyce, Joshua A.
author_facet Lundequist, Anders
Boyce, Joshua A.
author_sort Lundequist, Anders
collection PubMed
description BACKGROUND: Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. PRINCIPAL FINDINGS: Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. CONCLUSIONS: LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release.
format Text
id pubmed-3065470
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30654702011-04-04 LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release Lundequist, Anders Boyce, Joshua A. PLoS One Research Article BACKGROUND: Lysophosphatidic acid (LPA) is a bioactive lipid inducing proliferation, differentiation as well as cytokine release by mast cells through G-protein coupled receptors. Recently GPR92/LPA5 was identified as an LPA receptor highly expressed by cells of the immune system, which prompted us to investigate its presence and influence on mast cells. PRINCIPAL FINDINGS: Transcript analysis using quantitative real-time PCR revealed that LPA5 is the most prevalent LPA-receptor in human mast cells. Reduction of LPA5 levels using shRNA reduced calcium flux and abolished MIP-1β release in response to LPA. CONCLUSIONS: LPA5 is a bona fide LPA receptor on human mast cells responsible for the majority of LPA induced MIP-1β release. Public Library of Science 2011-03-28 /pmc/articles/PMC3065470/ /pubmed/21464938 http://dx.doi.org/10.1371/journal.pone.0018192 Text en Lundequist, Boyce. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lundequist, Anders
Boyce, Joshua A.
LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release
title LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release
title_full LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release
title_fullStr LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release
title_full_unstemmed LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release
title_short LPA5 Is Abundantly Expressed by Human Mast Cells and Important for Lysophosphatidic Acid Induced MIP-1β Release
title_sort lpa5 is abundantly expressed by human mast cells and important for lysophosphatidic acid induced mip-1β release
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3065470/
https://www.ncbi.nlm.nih.gov/pubmed/21464938
http://dx.doi.org/10.1371/journal.pone.0018192
work_keys_str_mv AT lundequistanders lpa5isabundantlyexpressedbyhumanmastcellsandimportantforlysophosphatidicacidinducedmip1brelease
AT boycejoshuaa lpa5isabundantlyexpressedbyhumanmastcellsandimportantforlysophosphatidicacidinducedmip1brelease

Ejemplares similares