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B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis

BACKGROUND: Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS: In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tu...

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Autores principales: Almeida, Luciana P, Trombone, Ana PF, Lorenzi, Julio CC, Rocha, Carolina D, Malardo, Thiago, Fontoura, Isabela C, Gembre, Ana F, Silva, Ricardo LL, Silva, Célio L, Castelo, Ademilson P, Coelho-Castelo, Arlete AM
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066104/
https://www.ncbi.nlm.nih.gov/pubmed/21401938
http://dx.doi.org/10.1186/1479-0556-9-5
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author Almeida, Luciana P
Trombone, Ana PF
Lorenzi, Julio CC
Rocha, Carolina D
Malardo, Thiago
Fontoura, Isabela C
Gembre, Ana F
Silva, Ricardo LL
Silva, Célio L
Castelo, Ademilson P
Coelho-Castelo, Arlete AM
author_facet Almeida, Luciana P
Trombone, Ana PF
Lorenzi, Julio CC
Rocha, Carolina D
Malardo, Thiago
Fontoura, Isabela C
Gembre, Ana F
Silva, Ricardo LL
Silva, Célio L
Castelo, Ademilson P
Coelho-Castelo, Arlete AM
author_sort Almeida, Luciana P
collection PubMed
description BACKGROUND: Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS: In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. RESULTS: In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. CONCLUSIONS: These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model.
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spelling pubmed-30661042011-03-30 B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis Almeida, Luciana P Trombone, Ana PF Lorenzi, Julio CC Rocha, Carolina D Malardo, Thiago Fontoura, Isabela C Gembre, Ana F Silva, Ricardo LL Silva, Célio L Castelo, Ademilson P Coelho-Castelo, Arlete AM Genet Vaccines Ther Research BACKGROUND: Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS: In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. RESULTS: In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. CONCLUSIONS: These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model. BioMed Central 2011-03-14 /pmc/articles/PMC3066104/ /pubmed/21401938 http://dx.doi.org/10.1186/1479-0556-9-5 Text en Copyright ©2011 Almeida et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Almeida, Luciana P
Trombone, Ana PF
Lorenzi, Julio CC
Rocha, Carolina D
Malardo, Thiago
Fontoura, Isabela C
Gembre, Ana F
Silva, Ricardo LL
Silva, Célio L
Castelo, Ademilson P
Coelho-Castelo, Arlete AM
B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
title B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
title_full B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
title_fullStr B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
title_full_unstemmed B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
title_short B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
title_sort b cells can modulate the cd8 memory t cell after dna vaccination against experimental tuberculosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066104/
https://www.ncbi.nlm.nih.gov/pubmed/21401938
http://dx.doi.org/10.1186/1479-0556-9-5
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