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B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis
BACKGROUND: Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS: In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tu...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066104/ https://www.ncbi.nlm.nih.gov/pubmed/21401938 http://dx.doi.org/10.1186/1479-0556-9-5 |
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author | Almeida, Luciana P Trombone, Ana PF Lorenzi, Julio CC Rocha, Carolina D Malardo, Thiago Fontoura, Isabela C Gembre, Ana F Silva, Ricardo LL Silva, Célio L Castelo, Ademilson P Coelho-Castelo, Arlete AM |
author_facet | Almeida, Luciana P Trombone, Ana PF Lorenzi, Julio CC Rocha, Carolina D Malardo, Thiago Fontoura, Isabela C Gembre, Ana F Silva, Ricardo LL Silva, Célio L Castelo, Ademilson P Coelho-Castelo, Arlete AM |
author_sort | Almeida, Luciana P |
collection | PubMed |
description | BACKGROUND: Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS: In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. RESULTS: In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. CONCLUSIONS: These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model. |
format | Text |
id | pubmed-3066104 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30661042011-03-30 B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis Almeida, Luciana P Trombone, Ana PF Lorenzi, Julio CC Rocha, Carolina D Malardo, Thiago Fontoura, Isabela C Gembre, Ana F Silva, Ricardo LL Silva, Célio L Castelo, Ademilson P Coelho-Castelo, Arlete AM Genet Vaccines Ther Research BACKGROUND: Although B cells are important as antigen presenting cells (APC) during the immune response, their role in DNA vaccination models is unknown. METHODS: In this study in vitro and in vivo experiments were performed to evaluate the ability of B cells to protect mice against Mycobacterium tuberculosis challenge. RESULTS: In vitro and in vivo studies showed that B cells efficiently present antigens after naked plasmid pcDNA3 encoding M. leprae 65-kDa heat shock protein (pcDNA3-Hsp65) internalization and protect B knock-out (BKO) mice against Mycobacterium tuberculosis infection. pcDNA3-Hsp65-transfected B cells adoptively transferred into BKO mice rescued the memory phenotypes and reduced the number of CFU compared to wild-type mice. CONCLUSIONS: These data not only suggest that B cells play an important role in the induction of CD8 T cells but also that they improve bacterial clearance in DNA vaccine model. BioMed Central 2011-03-14 /pmc/articles/PMC3066104/ /pubmed/21401938 http://dx.doi.org/10.1186/1479-0556-9-5 Text en Copyright ©2011 Almeida et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Almeida, Luciana P Trombone, Ana PF Lorenzi, Julio CC Rocha, Carolina D Malardo, Thiago Fontoura, Isabela C Gembre, Ana F Silva, Ricardo LL Silva, Célio L Castelo, Ademilson P Coelho-Castelo, Arlete AM B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis |
title | B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis |
title_full | B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis |
title_fullStr | B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis |
title_full_unstemmed | B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis |
title_short | B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis |
title_sort | b cells can modulate the cd8 memory t cell after dna vaccination against experimental tuberculosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066104/ https://www.ncbi.nlm.nih.gov/pubmed/21401938 http://dx.doi.org/10.1186/1479-0556-9-5 |
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