Cargando…

Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH)

BACKGROUND: Our current understanding of biofilms indicates that these structures are typically composed of many different microbial species. However, the lack of reliable techniques for the discrimination of each population has meant that studies focusing on multi-species biofilms are scarce and ty...

Descripción completa

Detalles Bibliográficos
Autores principales:	Almeida, Carina, Azevedo, Nuno F., Santos, Sílvio, Keevil, Charles W., Vieira, Maria J.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066202/ https://www.ncbi.nlm.nih.gov/pubmed/21479268 http://dx.doi.org/10.1371/journal.pone.0014786

Ejemplares similares

Correction: Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH)
por: Almeida, Carina, et al.
Publicado: (2013)

Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
por: Rocha, Rui, et al.
Publicado: (2018)

Correction: Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
por: Rocha, Rui, et al.
Publicado: (2018)

DNA Mimics for the Rapid Identification of Microorganisms by Fluorescence in situ Hybridization (FISH)
por: Cerqueira, Laura, et al.
Publicado: (2008)

Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.
por: Machado, António, et al.
Publicado: (2013)

Accelerated bacterial detection in blood culture by enhanced acoustic flow cytometry (AFC) following peptide nucleic acid fluorescence in situ hybridization (PNA-FISH)
por: Huang, Xiao Xuan, et al.
Publicado: (2019)

Optimizing locked nucleic acid/2’-O-methyl-RNA fluorescence in situ hybridization (LNA/2’OMe-FISH) procedure for bacterial detection
por: Azevedo, Andreia S., et al.
Publicado: (2019)

Spectral imaging and nucleic acid mimics fluorescence in situ hybridization (SI-NAM-FISH) for multiplex detection of clinical pathogens
por: Azevedo, Andreia S., et al.
Publicado: (2022)

Diagnosis of bacterial vaginosis by a new multiplex peptide nucleic acid fluorescence in situ hybridization method
por: Machado, António, et al.
Publicado: (2015)

Establishment of a New PNA-FISH Method for Aspergillus fumigatus Identification: First Insights for Future Use in Pulmonary Samples
por: Cerqueira, Laura, et al.
Publicado: (2020)

PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori
por: Cerqueira, Laura, et al.
Publicado: (2011)

Imaging biofilms using fluorescence in situ hybridization: seeing is believing
por: Barbosa, Ana, et al.
Publicado: (2023)

Development of a PNA Probe for Fluorescence In Situ Hybridization Detection of Prorocentrum donghaiense
por: Chen, Guofu, et al.
Publicado: (2011)

A New PNA-FISH Probe Targeting Fannyhessea vaginae
por: Sousa, Lúcia G. V., et al.
Publicado: (2021)

Improved Synthesis Strategy for Peptide Nucleic Acids (PNA) appropriate for Cell-specific Fluorescence Imaging
por: Pipkorn, Rüdiger, et al.
Publicado: (2011)

PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs
por: Jiang, Wenjun, et al.
Publicado: (2023)

Microfluidics combined with fluorescence in situ hybridization (FISH) for Candida spp. detection
por: Barbosa, Violina Baranauskaite, et al.
Publicado: (2022)

Rapid identification of Stenotrophomonas maltophilia by peptide nucleic acid fluorescence in situ hybridization
por: Hansen, N, et al.
Publicado: (2014)

γPNA FRET Pair Miniprobes for Quantitative Fluorescent In Situ Hybridization to Telomeric DNA in Cells and Tissue
por: Orenstein, Alexander, et al.
Publicado: (2017)

Effectiveness of blocking primers and a peptide nucleic acid (PNA) clamp for 18S metabarcoding dietary analysis of herbivorous fish
por: Homma, Chiho, et al.
Publicado: (2022)

Vitamin B(12) as a carrier of peptide nucleic acid (PNA) into bacterial cells
por: Równicki, Marcin, et al.
Publicado: (2017)

Cooperative Cellular Uptake and Activity of Octaarginine Antisense Peptide Nucleic Acid (PNA) Conjugates
por: Ghavami, Mahdi, et al.
Publicado: (2019)

Development of a Prevotella bivia PNA probe and a multiplex approach to detect three relevant species in bacterial vaginosis-associated biofilms
por: Sousa, Lúcia G. V., et al.
Publicado: (2023)

Fluorescence In Situ Hybridization (FISH) and Its Applications
por: Shakoori, Abdul Rauf
Publicado: (2017)

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications
por: Suparpprom, Chaturong, et al.
Publicado: (2022)

Diphenylalanine Motif Drives Self‐Assembling in Hybrid PNA‐Peptide Conjugates
por: Diaferia, Carlo, et al.
Publicado: (2021)

Unravelling the Bacterial Vaginosis-Associated Biofilm: A Multiplex Gardnerella vaginalis and Atopobium vaginae Fluorescence In Situ Hybridization Assay Using Peptide Nucleic Acid Probes
por: Hardy, Liselotte, et al.
Publicado: (2015)

Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization
por: Genet, Matthew D, et al.
Publicado: (2013)

Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
por: Sarrate, Zaida, et al.
Publicado: (2009)

Side chain modified peptide nucleic acids (PNA) for knock-down of six3 in medaka embryos
por: Dorn, Sebastian, et al.
Publicado: (2012)

Fluorescence Detection of KRAS2 mRNA Hybridization in Lung Cancer Cells with PNA-Peptides Containing an Internal Thiazole Orange
por: Sonar, Mahesh V., et al.
Publicado: (2014)

Gemdimethyl Peptide Nucleic Acids (α/β/γ-gdm-PNA): E/Z-Rotamers Influence the Selectivity in the Formation of Parallel/Antiparallel gdm-PNA:DNA/RNA Duplexes
por: Kulkarni, Pradnya, et al.
Publicado: (2022)

Cell-penetrating peptide conjugates of peptide nucleic acids (PNA) as inhibitors of HIV-1 Tat-dependent trans-activation in cells
por: Turner, John J., et al.
Publicado: (2005)

Inhibition of Multidrug Resistance by SV40 Pseudovirion Delivery of an Antigene Peptide Nucleic Acid (PNA) in Cultured Cells
por: Macadangdang, Benjamin, et al.
Publicado: (2011)

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
por: Blanco, Ana M., et al.
Publicado: (2009)

Peptide Nucleic Acid–Fluorescence In Situ Hybridization for Detection of Staphylococci From Endophthalmitis Isolates: A Proof-of-Concept Study
por: Patel, Nimesh, et al.
Publicado: (2017)

Genital mycobacteriosis caused by Mycobacterium marinum detected in two captive sharks by peptide nucleic acid–fluorescence in situ hybridization
por: Inohana, Mari, et al.
Publicado: (2022)

A Fluorescence In Situ Hybridization (FISH) Test for Diagnosing Babesiosis
por: Shah, Jyotsna S., et al.
Publicado: (2020)

Fluorescence in situ hybridization (FISH) and cell sorting of living bacteria
por: Batani, Giampiero, et al.
Publicado: (2019)

Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial Detection
por: Gu, Junjie, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_uodv07iqdjhj9aiscgmseh6231