Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice

SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog,...

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Autores principales: Suh, Young Ho, Yoshimoto-Furusawa, Aki, Weih, Karis A., Tessarollo, Lino, Roche, Katherine W., Mackem, Susan, Roche, Paul A.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066230/
https://www.ncbi.nlm.nih.gov/pubmed/21479242
http://dx.doi.org/10.1371/journal.pone.0018444
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author Suh, Young Ho
Yoshimoto-Furusawa, Aki
Weih, Karis A.
Tessarollo, Lino
Roche, Katherine W.
Mackem, Susan
Roche, Paul A.
author_facet Suh, Young Ho
Yoshimoto-Furusawa, Aki
Weih, Karis A.
Tessarollo, Lino
Roche, Katherine W.
Mackem, Susan
Roche, Paul A.
author_sort Suh, Young Ho
collection PubMed
description SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog, remains unknown. To reveal the importance of SNAP-23 function in mouse development, we generated Snap23-null mice by homologous recombination. We were unable to obtain newborn SNAP-23-deficient mice, and analysis of pre-implantation embryos from Snap23 (Δ/wt) matings revealed that Snap23-null blastocysts were dying prior to implantation at embryonic day E3.5. Thus these data reveal a critical role for SNAP-23 during embryogenesis.
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spelling pubmed-30662302011-04-08 Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice Suh, Young Ho Yoshimoto-Furusawa, Aki Weih, Karis A. Tessarollo, Lino Roche, Katherine W. Mackem, Susan Roche, Paul A. PLoS One Research Article SNARE-mediated membrane fusion is a pivotal event for a wide-variety of biological processes. SNAP-25, a neuron-specific SNARE protein, has been well-characterized and mouse embryos lacking Snap25 are viable. However, the phenotype of mice lacking SNAP-23, the ubiquitously expressed SNAP-25 homolog, remains unknown. To reveal the importance of SNAP-23 function in mouse development, we generated Snap23-null mice by homologous recombination. We were unable to obtain newborn SNAP-23-deficient mice, and analysis of pre-implantation embryos from Snap23 (Δ/wt) matings revealed that Snap23-null blastocysts were dying prior to implantation at embryonic day E3.5. Thus these data reveal a critical role for SNAP-23 during embryogenesis. Public Library of Science 2011-03-29 /pmc/articles/PMC3066230/ /pubmed/21479242 http://dx.doi.org/10.1371/journal.pone.0018444 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Suh, Young Ho
Yoshimoto-Furusawa, Aki
Weih, Karis A.
Tessarollo, Lino
Roche, Katherine W.
Mackem, Susan
Roche, Paul A.
Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice
title Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice
title_full Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice
title_fullStr Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice
title_full_unstemmed Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice
title_short Deletion of SNAP-23 Results in Pre-Implantation Embryonic Lethality in Mice
title_sort deletion of snap-23 results in pre-implantation embryonic lethality in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066230/
https://www.ncbi.nlm.nih.gov/pubmed/21479242
http://dx.doi.org/10.1371/journal.pone.0018444
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