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Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees
This study presents a molecular genetic characterization of Atlantic cod reared in commercial marine farms. Samples consisted of approximately 47 fish collected from nine cages located on four farms throughout Norway. In addition, 28 farmed escapees were recaptured in the sea (443 fish in total). Ni...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Blackwell Publishing Ltd
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068200/ https://www.ncbi.nlm.nih.gov/pubmed/20331613 http://dx.doi.org/10.1111/j.1365-2052.2010.02025.x |
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author | Glover, K A Dahle, G Westgaard, J I Johansen, T Knutsen, H Jørstad, K E |
author_facet | Glover, K A Dahle, G Westgaard, J I Johansen, T Knutsen, H Jørstad, K E |
author_sort | Glover, K A |
collection | PubMed |
description | This study presents a molecular genetic characterization of Atlantic cod reared in commercial marine farms. Samples consisted of approximately 47 fish collected from nine cages located on four farms throughout Norway. In addition, 28 farmed escapees were recaptured in the sea (443 fish in total). Nine microsatellite loci and the Pan I gene were analysed, revealing a total of 181 alleles. Each sample contained 43–63% of total allelic variation. Comparing variation with published data for wild cod indicates that lower genetic variation exists within single cages than in wild populations. Significant linkage disequilibrium was observed amongst pairs of loci in all samples, suggesting a low number of contributing parental fish. Global F(ST) was 0.049, and the highest pairwise F(ST) value (pooled loci) was 0.085. For single loci, the Pan I gene was the most diagnostic, displaying a global F(ST) of 0.203. Simulations amongst the samples collected on farms revealed an overall correct self-assignment percentage of 75%, demonstrating a high probability of identifying individuals to their farm of origin. Identification of the 28 escapees revealed a single cage as the most likely source of origin for half of the escapees, whilst the remaining fish were assigned to a mixture of samples, suggesting more than one source of escapees. |
format | Text |
id | pubmed-3068200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-30682002011-04-02 Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees Glover, K A Dahle, G Westgaard, J I Johansen, T Knutsen, H Jørstad, K E Anim Genet Articles This study presents a molecular genetic characterization of Atlantic cod reared in commercial marine farms. Samples consisted of approximately 47 fish collected from nine cages located on four farms throughout Norway. In addition, 28 farmed escapees were recaptured in the sea (443 fish in total). Nine microsatellite loci and the Pan I gene were analysed, revealing a total of 181 alleles. Each sample contained 43–63% of total allelic variation. Comparing variation with published data for wild cod indicates that lower genetic variation exists within single cages than in wild populations. Significant linkage disequilibrium was observed amongst pairs of loci in all samples, suggesting a low number of contributing parental fish. Global F(ST) was 0.049, and the highest pairwise F(ST) value (pooled loci) was 0.085. For single loci, the Pan I gene was the most diagnostic, displaying a global F(ST) of 0.203. Simulations amongst the samples collected on farms revealed an overall correct self-assignment percentage of 75%, demonstrating a high probability of identifying individuals to their farm of origin. Identification of the 28 escapees revealed a single cage as the most likely source of origin for half of the escapees, whilst the remaining fish were assigned to a mixture of samples, suggesting more than one source of escapees. Blackwell Publishing Ltd 2010-10 /pmc/articles/PMC3068200/ /pubmed/20331613 http://dx.doi.org/10.1111/j.1365-2052.2010.02025.x Text en Copyright © 2010 Stichting International Foundation for Animal Genetics http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Articles Glover, K A Dahle, G Westgaard, J I Johansen, T Knutsen, H Jørstad, K E Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
title | Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
title_full | Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
title_fullStr | Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
title_full_unstemmed | Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
title_short | Genetic diversity within and among Atlantic cod (Gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
title_sort | genetic diversity within and among atlantic cod (gadus morhua) farmed in marine cages: a proof-of-concept study for the identification of escapees |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068200/ https://www.ncbi.nlm.nih.gov/pubmed/20331613 http://dx.doi.org/10.1111/j.1365-2052.2010.02025.x |
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