Biochemical and Structural Characterization of Amy1: An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae

An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae was purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography. The enzyme was activated by NH(4)(+) and inhibited by Cu(+2) and Hg(+2). Signifi...

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Detalles Bibliográficos
Autores principales: Galdino, Alexsandro Sobreira, Silva, Roberto Nascimento, Lottermann, Muriele Taborda, Álvares, Alice Cunha Morales, de Moraes, Lídia Maria Pepe, Torres, Fernando Araripe Gonçalves, de Freitas, Sonia Maria, Ulhoa, Cirano José
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068306/
https://www.ncbi.nlm.nih.gov/pubmed/21490699
http://dx.doi.org/10.4061/2011/157294

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068306/
https://www.ncbi.nlm.nih.gov/pubmed/21490699
http://dx.doi.org/10.4061/2011/157294

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