Cargando…

Biochemical and Structural Characterization of Amy1: An Alpha-Amylase from Cryptococcus flavus Expressed in Saccharomyces cerevisiae

An extracellular alpha-amylase (Amy1) whose gene from Cryptococcus flavus was previously expressed in Saccharomyces cerevisiae was purified to homogeneity (67 kDa) by ion-exchange and molecular exclusion chromatography. The enzyme was activated by NH(4)(+) and inhibited by Cu(+2) and Hg(+2). Signifi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Galdino, Alexsandro Sobreira, Silva, Roberto Nascimento, Lottermann, Muriele Taborda, Álvares, Alice Cunha Morales, de Moraes, Lídia Maria Pepe, Torres, Fernando Araripe Gonçalves, de Freitas, Sonia Maria, Ulhoa, Cirano José
Formato:	Texto
Lenguaje:	English
Publicado:	SAGE-Hindawi Access to Research 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068306/ https://www.ncbi.nlm.nih.gov/pubmed/21490699 http://dx.doi.org/10.4061/2011/157294

Ejemplares similares

Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects
por: Moysés, Danuza Nogueira, et al.
Publicado: (2016)

Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
por: Viktor, Marko J, et al.
Publicado: (2013)

Promoter-proximal introns impact recombinant amylase expression in Saccharomyces cerevisiae
por: Schwerdtfeger, Kirstie S, et al.
Publicado: (2023)

Obesity, starch digestion and amylase: association between copy number variants at human salivary (AMY1) and pancreatic (AMY2) amylase genes
por: Carpenter, Danielle, et al.
Publicado: (2015)

A study on the use of strain-specific and homologous promoters for heterologous expression in industrial Saccharomyces cerevisiae strains
por: de Paiva, Daniel Pereira, et al.
Publicado: (2018)

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli
por: Pfrimer, Pollyana, et al.
Publicado: (2010)

A Recombinant Multiepitope Protein for Hepatitis B Diagnosis
por: de Souza, Marilen Queiroz, et al.
Publicado: (2013)

The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae
por: Martínez, José L., et al.
Publicado: (2016)

CRISPR/Cas9-mediated point mutations improve α-amylase secretion in Saccharomyces cerevisiae
por: Wang, Yanyan, et al.
Publicado: (2022)

Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain
por: Vilela, Leonardo de Figueiredo, et al.
Publicado: (2015)

Adaptive significance of amylase polymorphism in Drosophila. Analysis of the association between tissue-specific expression and specific activity in Amy(S )or Amy(F )genotypes of Drosophila subobscura
por: Terzić, Tatjana, et al.
Publicado: (1999)

Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae
por: Brain-Isasi, Stephanie, et al.
Publicado: (2017)

Measuring radiation-induced DNA damage in Cryptococcus neoformans and Saccharomyces cerevisiae using long range quantitative PCR
por: Cui, Wanchang, et al.
Publicado: (2018)

Potential Food Application of a Biosurfactant Produced by Saccharomyces cerevisiae URM 6670
por: Ribeiro, Beatriz Galdino, et al.
Publicado: (2020)

Comparison and characterization of α-amylase inducers in Aspergillus nidulans based on nuclear localization of AmyR
por: Murakoshi, Yuriko, et al.
Publicado: (2012)

Aspergillus nidulans AmyG Functions as an Intracellular α-Amylase to Promote α-Glucan Synthesis
por: Kazim, Alia Rizvi Syeda, et al.
Publicado: (2021)

Safety evaluation of the food enzyme maltogenic α‐amylase from the genetically modified Saccharomyces cerevisiae strain LALL‐MA
por: Silano, Vittorio, et al.
Publicado: (2021)

Functional Analysis of the P-Type ATPases Apt2-4 from Cryptococcus neoformans by Heterologous Expression in Saccharomyces cerevisiae
por: Veit, Sarina, et al.
Publicado: (2023)

Association of Salivary Amylase (AMY1) Gene Copy Number with Obesity in Alabama Elementary School Children
por: Venkatapoorna, Chandra M. K., et al.
Publicado: (2019)

Iron Regulatory Mechanisms in Saccharomyces cerevisiae
por: Ramos-Alonso, Lucía, et al.
Publicado: (2020)

Bel-ami /
por: Maupassant, Guy de

Post-Transcriptional Regulation of Iron Homeostasis in Saccharomyces cerevisiae
por: Martínez-Pastor, María Teresa, et al.
Publicado: (2013)

Bel-Ami
por: Maupassant, Guy de, 1850-1893

Bel-Ami
por: Maupassant, Guy de, 1850-1893

Bel Ami
por: Maupassant, Guy de, 1850-1893

Bel-Ami /
por: Maupassant, Guy de, 1850-1893
Publicado: (1993)

Bel-Ami /
por: Maupassant, Guy de, 1850-1893
Publicado: (1958)

Transcriptional profiling of Saccharomyces cerevisiae exposed to propolis
por: de Castro, Patrícia Alves, et al.
Publicado: (2012)

Saccharomyces cerevisiae and its industrial applications
por: Parapouli, Maria, et al.
Publicado: (2020)

Cryptococcus neoformans Prp8 Intein: An In Vivo Target-Based Drug Screening System in Saccharomyces cerevisiae to Identify Protein Splicing Inhibitors and Explore Its Dynamics
por: Fernandes, José Alex Lourenço, et al.
Publicado: (2022)

Switching the mode of sucrose utilization by Saccharomyces cerevisiae
por: Badotti, Fernanda, et al.
Publicado: (2008)

Individual Differences in AMY1 Gene Copy Number, Salivary α-Amylase Levels, and the Perception of Oral Starch
por: Mandel, Abigail L., et al.
Publicado: (2010)

Amy63, a novel type of marine bacterial multifunctional enzyme possessing amylase, agarase and carrageenase activities
por: Liu, Ge, et al.
Publicado: (2016)

Amylase activity is associated with AMY2B copy numbers in dog: implications for dog domestication, diet and diabetes
por: Arendt, Maja, et al.
Publicado: (2014)

Copy number variation of human AMY1 is a minor contributor to variation in salivary amylase expression and activity
por: Carpenter, Danielle, et al.
Publicado: (2017)

Expression of Paracoccidioides brasiliensis AMY1 in a Histoplasma capsulatum amy1 Mutant, Relates an α-(1,4)-Amylase to Cell Wall α-(1,3)-Glucan Synthesis
por: Camacho, Emma, et al.
Publicado: (2012)

Aneuploidy and Ethanol Tolerance in Saccharomyces cerevisiae
por: Morard, Miguel, et al.
Publicado: (2019)

Chemical Composition and Flavor Characteristics of Cider Fermented with Saccharomyces cerevisiae and Non-Saccharomyces cerevisiae
por: Wu, Yuzheng, et al.
Publicado: (2023)

TERRA and Telomere Maintenance in the Yeast Saccharomyces cerevisiae
por: Zeinoun, Bechara, et al.
Publicado: (2023)

Integrative Analysis of the Ethanol Tolerance of Saccharomyces cerevisiae
por: Wolf, Ivan Rodrigo, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_h9r0bd5tj2hap7mdr4bmnmem26