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Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function

Sperm membranes change in structure and composition upon ejaculation to undergo capacitation, a molecular transformation which enables spermatozoa to undergo the acrosome reaction and be capable of fertilization. Changes to the membrane environment including lipid composition, specifically lipid mic...

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Detalles Bibliográficos
Autores principales: Hickey, Katie D., Buhr, Mary M.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068514/
https://www.ncbi.nlm.nih.gov/pubmed/21490797
http://dx.doi.org/10.1155/2011/208457
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author Hickey, Katie D.
Buhr, Mary M.
author_facet Hickey, Katie D.
Buhr, Mary M.
author_sort Hickey, Katie D.
collection PubMed
description Sperm membranes change in structure and composition upon ejaculation to undergo capacitation, a molecular transformation which enables spermatozoa to undergo the acrosome reaction and be capable of fertilization. Changes to the membrane environment including lipid composition, specifically lipid microdomains, may be responsible for enabling capacitation. To study the effect of lipid environment on proteins, liposomes were created using lipids extracted from bull sperm membranes, with or without a protein (Na(+) K(+)-ATPase or α-amylase). Protein incorporation, function, and orientation were determined. Fluorescence resonance energy transfer (FRET) confirmed protein inclusion in the lipid bilayer, and protein function was confirmed using a colourometric assay of phosphate production from ATP cleavage. In the native lipid liposomes, ATPase was oriented with the β subunit facing the outer leaflet, while changing the lipid composition to 50% native lipids and 50% exogenous lipids significantly altered this orientation of Na(+) K(+)-ATPase within the membranes.
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spelling pubmed-30685142011-04-13 Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function Hickey, Katie D. Buhr, Mary M. J Lipids Research Article Sperm membranes change in structure and composition upon ejaculation to undergo capacitation, a molecular transformation which enables spermatozoa to undergo the acrosome reaction and be capable of fertilization. Changes to the membrane environment including lipid composition, specifically lipid microdomains, may be responsible for enabling capacitation. To study the effect of lipid environment on proteins, liposomes were created using lipids extracted from bull sperm membranes, with or without a protein (Na(+) K(+)-ATPase or α-amylase). Protein incorporation, function, and orientation were determined. Fluorescence resonance energy transfer (FRET) confirmed protein inclusion in the lipid bilayer, and protein function was confirmed using a colourometric assay of phosphate production from ATP cleavage. In the native lipid liposomes, ATPase was oriented with the β subunit facing the outer leaflet, while changing the lipid composition to 50% native lipids and 50% exogenous lipids significantly altered this orientation of Na(+) K(+)-ATPase within the membranes. Hindawi Publishing Corporation 2011 2011-01-26 /pmc/articles/PMC3068514/ /pubmed/21490797 http://dx.doi.org/10.1155/2011/208457 Text en Copyright © 2011 K. D. Hickey and M. M. Buhr. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hickey, Katie D.
Buhr, Mary M.
Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
title Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
title_full Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
title_fullStr Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
title_full_unstemmed Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
title_short Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function
title_sort lipid bilayer composition affects transmembrane protein orientation and function
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068514/
https://www.ncbi.nlm.nih.gov/pubmed/21490797
http://dx.doi.org/10.1155/2011/208457
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