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Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat

This study was aimed to investigate whether brain-derived neurotrophic factor (BDNF) can modulate local cerebral inflammation in ischemic stroke. Rats were subjected to ischemia by occluding the right middle cerebral artery (MCAO) for 2 hours. Rats were randomized as control, BDNF, and antibody grou...

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Detalles Bibliográficos
Autores principales: Jiang, Yongjun, Wei, Ning, Zhu, Juehua, Lu, Tingting, Chen, Zhaoyao, Xu, Gelin, Liu, Xinfeng
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068595/
https://www.ncbi.nlm.nih.gov/pubmed/21490702
http://dx.doi.org/10.1155/2010/372423
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author Jiang, Yongjun
Wei, Ning
Zhu, Juehua
Lu, Tingting
Chen, Zhaoyao
Xu, Gelin
Liu, Xinfeng
author_facet Jiang, Yongjun
Wei, Ning
Zhu, Juehua
Lu, Tingting
Chen, Zhaoyao
Xu, Gelin
Liu, Xinfeng
author_sort Jiang, Yongjun
collection PubMed
description This study was aimed to investigate whether brain-derived neurotrophic factor (BDNF) can modulate local cerebral inflammation in ischemic stroke. Rats were subjected to ischemia by occluding the right middle cerebral artery (MCAO) for 2 hours. Rats were randomized as control, BDNF, and antibody groups. The local inflammation was evaluated on cellular, cytokine, and transcription factor levels with immunofluorescence, enzyme-linked immunosorbent assay, real-time qPCR, and electrophoretic mobility shift assay, respectively. Exogenous BDNF significantly improved motor-sensory, sensorimotor function, and vestibulomotor function, while BDNF did not decrease the infarct volume. Exogenous BDNF increased the number of both activated and phagocytotic microglia in brain. BDNF upregulated interleukin10 and its mRNA expression, while downregulated tumor necrosis factor α and its mRNA expression. BDNF also increased DNA-binding activity of nuclear factor-kappa B. BDNF antibody, which blocked the activity of endogenous BDNF, showed the opposite effect of exogenous BDNF. Our data indicated that BDNF may modulate local inflammation in ischemic brain tissues on the cellular, cytokine, and transcription factor levels.
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spelling pubmed-30685952011-04-13 Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat Jiang, Yongjun Wei, Ning Zhu, Juehua Lu, Tingting Chen, Zhaoyao Xu, Gelin Liu, Xinfeng Mediators Inflamm Research Article This study was aimed to investigate whether brain-derived neurotrophic factor (BDNF) can modulate local cerebral inflammation in ischemic stroke. Rats were subjected to ischemia by occluding the right middle cerebral artery (MCAO) for 2 hours. Rats were randomized as control, BDNF, and antibody groups. The local inflammation was evaluated on cellular, cytokine, and transcription factor levels with immunofluorescence, enzyme-linked immunosorbent assay, real-time qPCR, and electrophoretic mobility shift assay, respectively. Exogenous BDNF significantly improved motor-sensory, sensorimotor function, and vestibulomotor function, while BDNF did not decrease the infarct volume. Exogenous BDNF increased the number of both activated and phagocytotic microglia in brain. BDNF upregulated interleukin10 and its mRNA expression, while downregulated tumor necrosis factor α and its mRNA expression. BDNF also increased DNA-binding activity of nuclear factor-kappa B. BDNF antibody, which blocked the activity of endogenous BDNF, showed the opposite effect of exogenous BDNF. Our data indicated that BDNF may modulate local inflammation in ischemic brain tissues on the cellular, cytokine, and transcription factor levels. Hindawi Publishing Corporation 2010 2011-02-24 /pmc/articles/PMC3068595/ /pubmed/21490702 http://dx.doi.org/10.1155/2010/372423 Text en Copyright © 2010 Yongjun Jiang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jiang, Yongjun
Wei, Ning
Zhu, Juehua
Lu, Tingting
Chen, Zhaoyao
Xu, Gelin
Liu, Xinfeng
Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
title Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
title_full Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
title_fullStr Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
title_full_unstemmed Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
title_short Effects of Brain-Derived Neurotrophic Factor on Local Inflammation in Experimental Stroke of Rat
title_sort effects of brain-derived neurotrophic factor on local inflammation in experimental stroke of rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068595/
https://www.ncbi.nlm.nih.gov/pubmed/21490702
http://dx.doi.org/10.1155/2010/372423
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