Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line

Haematophagous arthropods are the primary vectors in the transmission of Rickettsia, yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction between Ixodes...

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Detalles Bibliográficos
Autores principales: Thepparit, Chutima, Bourchookarn, Apichai, Petchampai, Natthida, Barker, Steven A., Macaluso, Kevin R.
Formato: Texto
Lenguaje:English
Publicado: Microbiology Society 2010
Materias:
Microbial Pathogenicity
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068691/
https://www.ncbi.nlm.nih.gov/pubmed/20558510
http://dx.doi.org/10.1099/mic.0.041400-0
Descripción
Sumario:Haematophagous arthropods are the primary vectors in the transmission of Rickettsia, yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction between Ixodes scapularis histone H2B and Rickettsia felis. Co-immunoprecipitation of histone with rickettsial cell lysate demonstrated the association of H2B with R. felis proteins, including outer-membrane protein B (OmpB), a major rickettsial adhesin molecule. The rickettsial infection of tick ISE6 cells was reduced by approximately 25 % via RNA-mediated H2B-depletion or enzymic treatment of histones. The interaction of H2B with the rickettsial adhesin OmpB suggests a role for H2B in mediating R. felis internalization into ISE6 cells.

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