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An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual

Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in hi...

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Autores principales: Garcia, Alan F., Abe, Lucienne M., Erdem, Guliz, Cortez, Chari L., Kurahara, David, Yamaga, Karen
Formato: Texto
Lenguaje:English
Publicado: Microbiology Society 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068697/
https://www.ncbi.nlm.nih.gov/pubmed/20634239
http://dx.doi.org/10.1099/mic.0.042614-0
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author Garcia, Alan F.
Abe, Lucienne M.
Erdem, Guliz
Cortez, Chari L.
Kurahara, David
Yamaga, Karen
author_facet Garcia, Alan F.
Abe, Lucienne M.
Erdem, Guliz
Cortez, Chari L.
Kurahara, David
Yamaga, Karen
author_sort Garcia, Alan F.
collection PubMed
description Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of S. pyogenes recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the covS gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional covS gene into the blood isolate resulted in high expression of CovS and expression of speB. These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow S. pyogenes to invade different niches in the body.
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spelling pubmed-30686972011-10-01 An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual Garcia, Alan F. Abe, Lucienne M. Erdem, Guliz Cortez, Chari L. Kurahara, David Yamaga, Karen Microbiology (Reading) Microbial Pathogenicity Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of S. pyogenes recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the covS gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional covS gene into the blood isolate resulted in high expression of CovS and expression of speB. These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow S. pyogenes to invade different niches in the body. Microbiology Society 2010-10 /pmc/articles/PMC3068697/ /pubmed/20634239 http://dx.doi.org/10.1099/mic.0.042614-0 Text en Copyright © 2010, SGM
spellingShingle Microbial Pathogenicity
Garcia, Alan F.
Abe, Lucienne M.
Erdem, Guliz
Cortez, Chari L.
Kurahara, David
Yamaga, Karen
An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
title An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
title_full An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
title_fullStr An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
title_full_unstemmed An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
title_short An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
title_sort insert in the covs gene distinguishes a pharyngeal and a blood isolate of streptococcus pyogenes found in the same individual
topic Microbial Pathogenicity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068697/
https://www.ncbi.nlm.nih.gov/pubmed/20634239
http://dx.doi.org/10.1099/mic.0.042614-0
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