Cargando…
An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in hi...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Microbiology Society
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068697/ https://www.ncbi.nlm.nih.gov/pubmed/20634239 http://dx.doi.org/10.1099/mic.0.042614-0 |
_version_ | 1782201276664119296 |
---|---|
author | Garcia, Alan F. Abe, Lucienne M. Erdem, Guliz Cortez, Chari L. Kurahara, David Yamaga, Karen |
author_facet | Garcia, Alan F. Abe, Lucienne M. Erdem, Guliz Cortez, Chari L. Kurahara, David Yamaga, Karen |
author_sort | Garcia, Alan F. |
collection | PubMed |
description | Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of S. pyogenes recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the covS gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional covS gene into the blood isolate resulted in high expression of CovS and expression of speB. These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow S. pyogenes to invade different niches in the body. |
format | Text |
id | pubmed-3068697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Microbiology Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-30686972011-10-01 An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual Garcia, Alan F. Abe, Lucienne M. Erdem, Guliz Cortez, Chari L. Kurahara, David Yamaga, Karen Microbiology (Reading) Microbial Pathogenicity Expression of the extensive arsenal of virulence factors by Streptococcus pyogenes is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of covRS arise spontaneously in vivo resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of S. pyogenes recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the covS gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional covS gene into the blood isolate resulted in high expression of CovS and expression of speB. These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow S. pyogenes to invade different niches in the body. Microbiology Society 2010-10 /pmc/articles/PMC3068697/ /pubmed/20634239 http://dx.doi.org/10.1099/mic.0.042614-0 Text en Copyright © 2010, SGM |
spellingShingle | Microbial Pathogenicity Garcia, Alan F. Abe, Lucienne M. Erdem, Guliz Cortez, Chari L. Kurahara, David Yamaga, Karen An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual |
title | An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual |
title_full | An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual |
title_fullStr | An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual |
title_full_unstemmed | An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual |
title_short | An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual |
title_sort | insert in the covs gene distinguishes a pharyngeal and a blood isolate of streptococcus pyogenes found in the same individual |
topic | Microbial Pathogenicity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068697/ https://www.ncbi.nlm.nih.gov/pubmed/20634239 http://dx.doi.org/10.1099/mic.0.042614-0 |
work_keys_str_mv | AT garciaalanf aninsertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT abeluciennem aninsertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT erdemguliz aninsertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT cortezcharil aninsertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT kuraharadavid aninsertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT yamagakaren aninsertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT garciaalanf insertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT abeluciennem insertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT erdemguliz insertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT cortezcharil insertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT kuraharadavid insertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual AT yamagakaren insertinthecovsgenedistinguishesapharyngealandabloodisolateofstreptococcuspyogenesfoundinthesameindividual |