Cargando…

The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals

The tight control of autolysis by Streptococcus mutans is critical for proper virulence gene expression and biofilm formation. A pair of dicistronic operons, SMU.575/574 (lrgAB) and SMU.1701/1700 (designated cidAB), encode putative membrane proteins that share structural features with the bacterioph...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahn, Sang-Joon, Rice, Kelly C., Oleas, Janneth, Bayles, Kenneth W., Burne, Robert A.
Formato: Texto
Lenguaje:English
Publicado: Microbiology Society 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068699/
https://www.ncbi.nlm.nih.gov/pubmed/20671018
http://dx.doi.org/10.1099/mic.0.039586-0
_version_ 1782201277135978496
author Ahn, Sang-Joon
Rice, Kelly C.
Oleas, Janneth
Bayles, Kenneth W.
Burne, Robert A.
author_facet Ahn, Sang-Joon
Rice, Kelly C.
Oleas, Janneth
Bayles, Kenneth W.
Burne, Robert A.
author_sort Ahn, Sang-Joon
collection PubMed
description The tight control of autolysis by Streptococcus mutans is critical for proper virulence gene expression and biofilm formation. A pair of dicistronic operons, SMU.575/574 (lrgAB) and SMU.1701/1700 (designated cidAB), encode putative membrane proteins that share structural features with the bacteriophage-encoded holin family of proteins, which modulate host cell lysis during lytic infection. Analysis of S. mutans lrg and cid mutants revealed a role for these operons in autolysis, biofilm formation, glucosyltransferase expression and oxidative stress tolerance. Expression of lrgAB was repressed during early exponential phase and was induced over 1000-fold as cells entered late exponential phase, whereas cidAB expression declined from early to late exponential phase. A two-component system encoded immediately upstream of lrgAB (LytST) was required for activation of lrgAB expression, but not for cid expression. In addition to availability of oxygen, glucose levels were revealed to affect lrg and cid transcription differentially and significantly, probably through CcpA (carbon catabolite protein A). Collectively, these findings demonstrate that the Cid/Lrg system can affect several virulence traits of S. mutans, and its expression is controlled by two major environmental signals, oxygen and glucose. Moreover, cid/lrg expression is tightly regulated by LytST and CcpA.
format Text
id pubmed-3068699
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Microbiology Society
record_format MEDLINE/PubMed
spelling pubmed-30686992011-10-01 The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals Ahn, Sang-Joon Rice, Kelly C. Oleas, Janneth Bayles, Kenneth W. Burne, Robert A. Microbiology (Reading) Microbial Pathogenicity The tight control of autolysis by Streptococcus mutans is critical for proper virulence gene expression and biofilm formation. A pair of dicistronic operons, SMU.575/574 (lrgAB) and SMU.1701/1700 (designated cidAB), encode putative membrane proteins that share structural features with the bacteriophage-encoded holin family of proteins, which modulate host cell lysis during lytic infection. Analysis of S. mutans lrg and cid mutants revealed a role for these operons in autolysis, biofilm formation, glucosyltransferase expression and oxidative stress tolerance. Expression of lrgAB was repressed during early exponential phase and was induced over 1000-fold as cells entered late exponential phase, whereas cidAB expression declined from early to late exponential phase. A two-component system encoded immediately upstream of lrgAB (LytST) was required for activation of lrgAB expression, but not for cid expression. In addition to availability of oxygen, glucose levels were revealed to affect lrg and cid transcription differentially and significantly, probably through CcpA (carbon catabolite protein A). Collectively, these findings demonstrate that the Cid/Lrg system can affect several virulence traits of S. mutans, and its expression is controlled by two major environmental signals, oxygen and glucose. Moreover, cid/lrg expression is tightly regulated by LytST and CcpA. Microbiology Society 2010-10 /pmc/articles/PMC3068699/ /pubmed/20671018 http://dx.doi.org/10.1099/mic.0.039586-0 Text en Copyright © 2010, SGM
spellingShingle Microbial Pathogenicity
Ahn, Sang-Joon
Rice, Kelly C.
Oleas, Janneth
Bayles, Kenneth W.
Burne, Robert A.
The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals
title The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals
title_full The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals
title_fullStr The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals
title_full_unstemmed The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals
title_short The Streptococcus mutans Cid and Lrg systems modulate virulence traits in response to multiple environmental signals
title_sort streptococcus mutans cid and lrg systems modulate virulence traits in response to multiple environmental signals
topic Microbial Pathogenicity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3068699/
https://www.ncbi.nlm.nih.gov/pubmed/20671018
http://dx.doi.org/10.1099/mic.0.039586-0
work_keys_str_mv AT ahnsangjoon thestreptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT ricekellyc thestreptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT oleasjanneth thestreptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT bayleskennethw thestreptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT burneroberta thestreptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT ahnsangjoon streptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT ricekellyc streptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT oleasjanneth streptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT bayleskennethw streptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals
AT burneroberta streptococcusmutanscidandlrgsystemsmodulatevirulencetraitsinresponsetomultipleenvironmentalsignals