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IFT20 is required for opsin trafficking and photoreceptor outer segment development

The light-detecting outer segments of vertebrate photoreceptors are cilia. Like other cilia, all materials needed for assembly and maintenance are synthesized in the cell body and transported into the cilium. The highly elaborated nature of the outer segment and its high rate of turnover necessitate...

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Detalles Bibliográficos
Autores principales: Keady, Brian T., Le, Yun Zheng, Pazour, Gregory J.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069017/
https://www.ncbi.nlm.nih.gov/pubmed/21307337
http://dx.doi.org/10.1091/mbc.E10-09-0792
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author Keady, Brian T.
Le, Yun Zheng
Pazour, Gregory J.
author_facet Keady, Brian T.
Le, Yun Zheng
Pazour, Gregory J.
author_sort Keady, Brian T.
collection PubMed
description The light-detecting outer segments of vertebrate photoreceptors are cilia. Like other cilia, all materials needed for assembly and maintenance are synthesized in the cell body and transported into the cilium. The highly elaborated nature of the outer segment and its high rate of turnover necessitate unusually high levels of transport into the cilium. In this work, we examine the role of the IFT20 subunit of the intraflagellar transport (IFT) particle in photoreceptor cells. IFT20 was deleted in developing cones by a cone-specific Cre and in mature rods and cones by a tamoxifen-activatable Cre. Loss of IFT20 during cone development leads to opsin accumulation in the inner segment even when the connecting cilium and outer segment are still intact. With time this causes cone cell degeneration. Similarly, deletion of IFT20 in mature rods causes rapid accumulation of rhodopsin in the cell body, where it is concentrated at the Golgi complex. We further show that IFT20, acting both as part of the IFT particle and independent of the particle, binds to rhodopsin and RG-opsin. Since IFT20 dynamically moves between the Golgi complex and the connecting cilium, the current work suggests that rhodopsin and opsins are cargo for IFT transport.
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spelling pubmed-30690172011-06-16 IFT20 is required for opsin trafficking and photoreceptor outer segment development Keady, Brian T. Le, Yun Zheng Pazour, Gregory J. Mol Biol Cell Articles The light-detecting outer segments of vertebrate photoreceptors are cilia. Like other cilia, all materials needed for assembly and maintenance are synthesized in the cell body and transported into the cilium. The highly elaborated nature of the outer segment and its high rate of turnover necessitate unusually high levels of transport into the cilium. In this work, we examine the role of the IFT20 subunit of the intraflagellar transport (IFT) particle in photoreceptor cells. IFT20 was deleted in developing cones by a cone-specific Cre and in mature rods and cones by a tamoxifen-activatable Cre. Loss of IFT20 during cone development leads to opsin accumulation in the inner segment even when the connecting cilium and outer segment are still intact. With time this causes cone cell degeneration. Similarly, deletion of IFT20 in mature rods causes rapid accumulation of rhodopsin in the cell body, where it is concentrated at the Golgi complex. We further show that IFT20, acting both as part of the IFT particle and independent of the particle, binds to rhodopsin and RG-opsin. Since IFT20 dynamically moves between the Golgi complex and the connecting cilium, the current work suggests that rhodopsin and opsins are cargo for IFT transport. The American Society for Cell Biology 2011-04-01 /pmc/articles/PMC3069017/ /pubmed/21307337 http://dx.doi.org/10.1091/mbc.E10-09-0792 Text en © 2011 Keady et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Keady, Brian T.
Le, Yun Zheng
Pazour, Gregory J.
IFT20 is required for opsin trafficking and photoreceptor outer segment development
title IFT20 is required for opsin trafficking and photoreceptor outer segment development
title_full IFT20 is required for opsin trafficking and photoreceptor outer segment development
title_fullStr IFT20 is required for opsin trafficking and photoreceptor outer segment development
title_full_unstemmed IFT20 is required for opsin trafficking and photoreceptor outer segment development
title_short IFT20 is required for opsin trafficking and photoreceptor outer segment development
title_sort ift20 is required for opsin trafficking and photoreceptor outer segment development
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069017/
https://www.ncbi.nlm.nih.gov/pubmed/21307337
http://dx.doi.org/10.1091/mbc.E10-09-0792
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