Cargando…

Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study

BACKGROUND: Thoracic insufficiency syndrome represents a novel form of postnatal restrictive respiratory disease occurring in children with early-onset scoliosis and chest wall anomalies. Expansion thoracoplasty improves lung volumes in children with thoracic insufficiency syndrome; however, how it...

Descripción completa

Detalles Bibliográficos
Autores principales: Olson, J. Casey, Kurek, Kyle C., Mehta, Hemal P., Warman, Matt L., Snyder, Brian D.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069277/
https://www.ncbi.nlm.nih.gov/pubmed/21424837
http://dx.doi.org/10.1007/s11999-011-1807-0
_version_ 1782201330406785024
author Olson, J. Casey
Kurek, Kyle C.
Mehta, Hemal P.
Warman, Matt L.
Snyder, Brian D.
author_facet Olson, J. Casey
Kurek, Kyle C.
Mehta, Hemal P.
Warman, Matt L.
Snyder, Brian D.
author_sort Olson, J. Casey
collection PubMed
description BACKGROUND: Thoracic insufficiency syndrome represents a novel form of postnatal restrictive respiratory disease occurring in children with early-onset scoliosis and chest wall anomalies. Expansion thoracoplasty improves lung volumes in children with thoracic insufficiency syndrome; however, how it affects lung development is unknown. QUESTIONS/PURPOSES: Using a rabbit model of thoracic insufficiency syndrome, we evaluated the effect of expansion thoracoplasty on the response of biologic mechanisms in the alveolar microstructure. METHODS: Using archived material from a previous experiment, 10 4-week-old New Zealand rabbits were divided into three groups: normal (n = 3), disease (n = 3), and treated (n = 4). Left ribs four to eight were tethered in seven rabbits at age 5 weeks to induce hypoplasia of the left hemithorax (disease). At age 10 weeks, four of these rabbits were treated by expansion thoracoplasty (treated). At age 24 weeks, lungs were excised and processed. Alveolar density and parenchymal airspace were measured on histologic sections. Immunohistochemistry was performed for vascular endothelial growth factor receptor 2 (angiogenesis), KI-67 (cell proliferation), and RAM-11 (macrophages). RESULTS: Alveolar walls were poorly perfused and airspace fraction was larger (emphysematous) in disease rabbits than normal or treated rabbits. Immunohistochemistry provided inconclusive evidence to support the concept that pulmonary hypoplasia is induced by thoracic insufficiency syndrome and controlled by expansion thoracoplasty. CONCLUSIONS: Treatment of thoracic insufficiency syndrome by expansion thoracoplasty may prevent emphysematous changes in the alveolar microstructure, thereby enhancing gas exchange. CLINICAL RELEVANCE: Creating an animal model for thoracic insufficiency syndrome should provide insight into the effect of expansion thoracoplasty on lung development otherwise clinically unattainable.
format Text
id pubmed-3069277
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-30692772011-05-02 Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study Olson, J. Casey Kurek, Kyle C. Mehta, Hemal P. Warman, Matt L. Snyder, Brian D. Clin Orthop Relat Res Symposium: Early Onset Scoliosis BACKGROUND: Thoracic insufficiency syndrome represents a novel form of postnatal restrictive respiratory disease occurring in children with early-onset scoliosis and chest wall anomalies. Expansion thoracoplasty improves lung volumes in children with thoracic insufficiency syndrome; however, how it affects lung development is unknown. QUESTIONS/PURPOSES: Using a rabbit model of thoracic insufficiency syndrome, we evaluated the effect of expansion thoracoplasty on the response of biologic mechanisms in the alveolar microstructure. METHODS: Using archived material from a previous experiment, 10 4-week-old New Zealand rabbits were divided into three groups: normal (n = 3), disease (n = 3), and treated (n = 4). Left ribs four to eight were tethered in seven rabbits at age 5 weeks to induce hypoplasia of the left hemithorax (disease). At age 10 weeks, four of these rabbits were treated by expansion thoracoplasty (treated). At age 24 weeks, lungs were excised and processed. Alveolar density and parenchymal airspace were measured on histologic sections. Immunohistochemistry was performed for vascular endothelial growth factor receptor 2 (angiogenesis), KI-67 (cell proliferation), and RAM-11 (macrophages). RESULTS: Alveolar walls were poorly perfused and airspace fraction was larger (emphysematous) in disease rabbits than normal or treated rabbits. Immunohistochemistry provided inconclusive evidence to support the concept that pulmonary hypoplasia is induced by thoracic insufficiency syndrome and controlled by expansion thoracoplasty. CONCLUSIONS: Treatment of thoracic insufficiency syndrome by expansion thoracoplasty may prevent emphysematous changes in the alveolar microstructure, thereby enhancing gas exchange. CLINICAL RELEVANCE: Creating an animal model for thoracic insufficiency syndrome should provide insight into the effect of expansion thoracoplasty on lung development otherwise clinically unattainable. Springer-Verlag 2011-03-22 2011-05 /pmc/articles/PMC3069277/ /pubmed/21424837 http://dx.doi.org/10.1007/s11999-011-1807-0 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Symposium: Early Onset Scoliosis
Olson, J. Casey
Kurek, Kyle C.
Mehta, Hemal P.
Warman, Matt L.
Snyder, Brian D.
Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study
title Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study
title_full Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study
title_fullStr Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study
title_full_unstemmed Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study
title_short Expansion Thoracoplasty Affects Lung Growth and Morphology in a Rabbit Model: A Pilot Study
title_sort expansion thoracoplasty affects lung growth and morphology in a rabbit model: a pilot study
topic Symposium: Early Onset Scoliosis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069277/
https://www.ncbi.nlm.nih.gov/pubmed/21424837
http://dx.doi.org/10.1007/s11999-011-1807-0
work_keys_str_mv AT olsonjcasey expansionthoracoplastyaffectslunggrowthandmorphologyinarabbitmodelapilotstudy
AT kurekkylec expansionthoracoplastyaffectslunggrowthandmorphologyinarabbitmodelapilotstudy
AT mehtahemalp expansionthoracoplastyaffectslunggrowthandmorphologyinarabbitmodelapilotstudy
AT warmanmattl expansionthoracoplastyaffectslunggrowthandmorphologyinarabbitmodelapilotstudy
AT snyderbriand expansionthoracoplastyaffectslunggrowthandmorphologyinarabbitmodelapilotstudy