Next generation high density self assembling functional protein arrays

We report a high-density self assembling protein microarray that displays thousands of proteins, produced and captured in situ from immobilized cDNA templates. Over 1500 unique cDNAs were tested with > 90% success with nearly all proteins displaying yields within 2 fold of the mean, minimal sampl...

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Detalles Bibliográficos
Autores principales: Ramachandran, Niroshan, Raphael, Jacob V., Hainsworth, Eugenie, Demirkan, Gokhan, Fuentes, Manuel G., Rolfs, Andreas, Hu, Yanhui, LaBaer, Joshua
Formato: Texto
Lenguaje:English
Publicado: 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070491/
https://www.ncbi.nlm.nih.gov/pubmed/18469824
http://dx.doi.org/10.1038/nmeth.1210
Descripción
Sumario:We report a high-density self assembling protein microarray that displays thousands of proteins, produced and captured in situ from immobilized cDNA templates. Over 1500 unique cDNAs were tested with > 90% success with nearly all proteins displaying yields within 2 fold of the mean, minimal sample variation and good day to day reproducibility. The displayed proteins revealed selective protein interactions. This method will enable various experimental approaches to study protein function in high throughput.

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