Next generation high density self assembling functional protein arrays

We report a high-density self assembling protein microarray that displays thousands of proteins, produced and captured in situ from immobilized cDNA templates. Over 1500 unique cDNAs were tested with > 90% success with nearly all proteins displaying yields within 2 fold of the mean, minimal sampl...

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Detalles Bibliográficos
Autores principales: Ramachandran, Niroshan, Raphael, Jacob V., Hainsworth, Eugenie, Demirkan, Gokhan, Fuentes, Manuel G., Rolfs, Andreas, Hu, Yanhui, LaBaer, Joshua
Formato: Texto
Lenguaje:English
Publicado: 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070491/
https://www.ncbi.nlm.nih.gov/pubmed/18469824
http://dx.doi.org/10.1038/nmeth.1210
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author Ramachandran, Niroshan
Raphael, Jacob V.
Hainsworth, Eugenie
Demirkan, Gokhan
Fuentes, Manuel G.
Rolfs, Andreas
Hu, Yanhui
LaBaer, Joshua
author_facet Ramachandran, Niroshan
Raphael, Jacob V.
Hainsworth, Eugenie
Demirkan, Gokhan
Fuentes, Manuel G.
Rolfs, Andreas
Hu, Yanhui
LaBaer, Joshua
author_sort Ramachandran, Niroshan
collection PubMed
description We report a high-density self assembling protein microarray that displays thousands of proteins, produced and captured in situ from immobilized cDNA templates. Over 1500 unique cDNAs were tested with > 90% success with nearly all proteins displaying yields within 2 fold of the mean, minimal sample variation and good day to day reproducibility. The displayed proteins revealed selective protein interactions. This method will enable various experimental approaches to study protein function in high throughput.
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spelling pubmed-30704912011-04-04 Next generation high density self assembling functional protein arrays Ramachandran, Niroshan Raphael, Jacob V. Hainsworth, Eugenie Demirkan, Gokhan Fuentes, Manuel G. Rolfs, Andreas Hu, Yanhui LaBaer, Joshua Nat Methods Article We report a high-density self assembling protein microarray that displays thousands of proteins, produced and captured in situ from immobilized cDNA templates. Over 1500 unique cDNAs were tested with > 90% success with nearly all proteins displaying yields within 2 fold of the mean, minimal sample variation and good day to day reproducibility. The displayed proteins revealed selective protein interactions. This method will enable various experimental approaches to study protein function in high throughput. 2008-05-11 2008-06 /pmc/articles/PMC3070491/ /pubmed/18469824 http://dx.doi.org/10.1038/nmeth.1210 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ramachandran, Niroshan
Raphael, Jacob V.
Hainsworth, Eugenie
Demirkan, Gokhan
Fuentes, Manuel G.
Rolfs, Andreas
Hu, Yanhui
LaBaer, Joshua
Next generation high density self assembling functional protein arrays
title Next generation high density self assembling functional protein arrays
title_full Next generation high density self assembling functional protein arrays
title_fullStr Next generation high density self assembling functional protein arrays
title_full_unstemmed Next generation high density self assembling functional protein arrays
title_short Next generation high density self assembling functional protein arrays
title_sort next generation high density self assembling functional protein arrays
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070491/
https://www.ncbi.nlm.nih.gov/pubmed/18469824
http://dx.doi.org/10.1038/nmeth.1210
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