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Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity

BACKGROUND: Adipose tissue abundance relies partly on the factors that regulate adipogenesis, i.e. proliferation and differentiation of adipocytes. While components of the transcriptional program that initiates adipogenesis is well-known, the importance of microRNAs in adipogenesis is less well stud...

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Autores principales: Keller, Pernille, Gburcik, Valentina, Petrovic, Natasa, Gallagher, Iain J, Nedergaard, Jan, Cannon, Barbara, Timmons, James A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070678/
https://www.ncbi.nlm.nih.gov/pubmed/21426570
http://dx.doi.org/10.1186/1472-6823-11-7
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author Keller, Pernille
Gburcik, Valentina
Petrovic, Natasa
Gallagher, Iain J
Nedergaard, Jan
Cannon, Barbara
Timmons, James A
author_facet Keller, Pernille
Gburcik, Valentina
Petrovic, Natasa
Gallagher, Iain J
Nedergaard, Jan
Cannon, Barbara
Timmons, James A
author_sort Keller, Pernille
collection PubMed
description BACKGROUND: Adipose tissue abundance relies partly on the factors that regulate adipogenesis, i.e. proliferation and differentiation of adipocytes. While components of the transcriptional program that initiates adipogenesis is well-known, the importance of microRNAs in adipogenesis is less well studied. We thus set out to investigate whether miRNAs would be actively modulated during adipogenesis and obesity. METHODS: Several models exist to study adipogenesis in vitro, of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. Thus, as an alternative, we produced EXIQON microarray of brown and white primary murine adipocytes (prior to and following differentiation) to yield global profiles of miRNAs. RESULTS: We found 65 miRNAs regulated during in vitro adipogenesis in primary adipocytes. We evaluated the similarity of our responses to those found in non-primary cell models, through literature data-mining. When comparing primary adipocyte profiles, with those of cell lines reported in the literature, we found a high degree of difference in 'adipogenesis' regulated miRNAs suggesting that the model systems may not be accurately representing adipogenesis. The expression of 10 adipogenesis-regulated miRNAs were studied using real-time qPCR and then we selected 5 miRNAs, that showed robust expression, were profiled in subcutaneous adipose tissue obtained from 20 humans with a range of body mass indices (BMI, range = 21-48, and all samples have U133+2 Affymetrix profiles provided). Of the miRNAs tested, mir-21 was robustly expressed in human adipose tissue and positively correlated with BMI (R2 = 0.49, p < 0.001). CONCLUSION: In conclusion, we provide a preliminary analysis of miRNAs associated with primary cell in vitro adipogenesis and demonstrate that the inflammation-associated miRNA, mir-21 is up-regulated in subcutaneous adipose tissue in human obesity. Further, we provide a novel transcriptomics database of EXIQON and Affymetrix adipocyte profiles to facilitate data mining.
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spelling pubmed-30706782011-04-05 Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity Keller, Pernille Gburcik, Valentina Petrovic, Natasa Gallagher, Iain J Nedergaard, Jan Cannon, Barbara Timmons, James A BMC Endocr Disord Research Article BACKGROUND: Adipose tissue abundance relies partly on the factors that regulate adipogenesis, i.e. proliferation and differentiation of adipocytes. While components of the transcriptional program that initiates adipogenesis is well-known, the importance of microRNAs in adipogenesis is less well studied. We thus set out to investigate whether miRNAs would be actively modulated during adipogenesis and obesity. METHODS: Several models exist to study adipogenesis in vitro, of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. Thus, as an alternative, we produced EXIQON microarray of brown and white primary murine adipocytes (prior to and following differentiation) to yield global profiles of miRNAs. RESULTS: We found 65 miRNAs regulated during in vitro adipogenesis in primary adipocytes. We evaluated the similarity of our responses to those found in non-primary cell models, through literature data-mining. When comparing primary adipocyte profiles, with those of cell lines reported in the literature, we found a high degree of difference in 'adipogenesis' regulated miRNAs suggesting that the model systems may not be accurately representing adipogenesis. The expression of 10 adipogenesis-regulated miRNAs were studied using real-time qPCR and then we selected 5 miRNAs, that showed robust expression, were profiled in subcutaneous adipose tissue obtained from 20 humans with a range of body mass indices (BMI, range = 21-48, and all samples have U133+2 Affymetrix profiles provided). Of the miRNAs tested, mir-21 was robustly expressed in human adipose tissue and positively correlated with BMI (R2 = 0.49, p < 0.001). CONCLUSION: In conclusion, we provide a preliminary analysis of miRNAs associated with primary cell in vitro adipogenesis and demonstrate that the inflammation-associated miRNA, mir-21 is up-regulated in subcutaneous adipose tissue in human obesity. Further, we provide a novel transcriptomics database of EXIQON and Affymetrix adipocyte profiles to facilitate data mining. BioMed Central 2011-03-22 /pmc/articles/PMC3070678/ /pubmed/21426570 http://dx.doi.org/10.1186/1472-6823-11-7 Text en Copyright ©2011 Keller et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Keller, Pernille
Gburcik, Valentina
Petrovic, Natasa
Gallagher, Iain J
Nedergaard, Jan
Cannon, Barbara
Timmons, James A
Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
title Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
title_full Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
title_fullStr Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
title_full_unstemmed Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
title_short Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity
title_sort gene-chip studies of adipogenesis-regulated micrornas in mouse primary adipocytes and human obesity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3070678/
https://www.ncbi.nlm.nih.gov/pubmed/21426570
http://dx.doi.org/10.1186/1472-6823-11-7
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