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Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation

BACKGROUND: Sewage workers are exposed to multiple chemicals among which many are suspected genotoxicants. Therefore, they might incur DNA damage and oxidative stress. We aimed to explore integrated urinary biomarkers, assessing the overall urine genotoxicity by in vitro comet and micronucleus assay...

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Autores principales: Al Zabadi, Hamzeh, Ferrari, Luc, Sari-Minodier, Irène, Kerautret, Marie-Aude, Tiberguent, Aziz, Paris, Christophe, Zmirou-Navier, Denis
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071309/
https://www.ncbi.nlm.nih.gov/pubmed/21435260
http://dx.doi.org/10.1186/1476-069X-10-23
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author Al Zabadi, Hamzeh
Ferrari, Luc
Sari-Minodier, Irène
Kerautret, Marie-Aude
Tiberguent, Aziz
Paris, Christophe
Zmirou-Navier, Denis
author_facet Al Zabadi, Hamzeh
Ferrari, Luc
Sari-Minodier, Irène
Kerautret, Marie-Aude
Tiberguent, Aziz
Paris, Christophe
Zmirou-Navier, Denis
author_sort Al Zabadi, Hamzeh
collection PubMed
description BACKGROUND: Sewage workers are exposed to multiple chemicals among which many are suspected genotoxicants. Therefore, they might incur DNA damage and oxidative stress. We aimed to explore integrated urinary biomarkers, assessing the overall urine genotoxicity by in vitro comet and micronucleus assays and measuring urinary 8-oxo-2'-deoxyguanosine. METHODS: During three consecutive working days, polycyclic aromatic hydrocarbons and volatile organic compounds were sampled in workplace air of 34 sewage and 30 office workers, as indicators of airborne exposure. The last day, subjects collected their 24 hours urine. Genotoxicity of urinary extracts was assessed by comet and micronucleus assays on a HepG2 cell line. Using competitive enzymatic immunoassay we evaluated the 24 hours urinary 8-oxo-2'-deoxyguanosine excretion. Benzo(a)pyrene toxicity equivalent factors and inhalation unit risk for Benzo(a)pyrene and benzene were used to give an estimate of cancer risk levels. RESULTS: Workplace air concentrations of polycyclic aromatic hydrocarbons (e.g. 23.7 [range 2.4-104.6] ng.m(-3 )for fluoranthene) and volatile organic compounds (e.g. 19.1 ± 2.9 [standard error] μ.m(-3 )for benzene) were elevated in sewage compared to office workplaces (P < 0.01) and corresponded to an increased lifetime cancer risk. The urinary extracts of sewage workers showed higher genotoxicity (P < 0.001) than office workers. CONCLUSIONS: The integrated and non-specific urinary biomarkers of exposure showed that sewage workers experience exposure to mixtures of genotoxicants in the workplace.
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spelling pubmed-30713092011-04-06 Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation Al Zabadi, Hamzeh Ferrari, Luc Sari-Minodier, Irène Kerautret, Marie-Aude Tiberguent, Aziz Paris, Christophe Zmirou-Navier, Denis Environ Health Research BACKGROUND: Sewage workers are exposed to multiple chemicals among which many are suspected genotoxicants. Therefore, they might incur DNA damage and oxidative stress. We aimed to explore integrated urinary biomarkers, assessing the overall urine genotoxicity by in vitro comet and micronucleus assays and measuring urinary 8-oxo-2'-deoxyguanosine. METHODS: During three consecutive working days, polycyclic aromatic hydrocarbons and volatile organic compounds were sampled in workplace air of 34 sewage and 30 office workers, as indicators of airborne exposure. The last day, subjects collected their 24 hours urine. Genotoxicity of urinary extracts was assessed by comet and micronucleus assays on a HepG2 cell line. Using competitive enzymatic immunoassay we evaluated the 24 hours urinary 8-oxo-2'-deoxyguanosine excretion. Benzo(a)pyrene toxicity equivalent factors and inhalation unit risk for Benzo(a)pyrene and benzene were used to give an estimate of cancer risk levels. RESULTS: Workplace air concentrations of polycyclic aromatic hydrocarbons (e.g. 23.7 [range 2.4-104.6] ng.m(-3 )for fluoranthene) and volatile organic compounds (e.g. 19.1 ± 2.9 [standard error] μ.m(-3 )for benzene) were elevated in sewage compared to office workplaces (P < 0.01) and corresponded to an increased lifetime cancer risk. The urinary extracts of sewage workers showed higher genotoxicity (P < 0.001) than office workers. CONCLUSIONS: The integrated and non-specific urinary biomarkers of exposure showed that sewage workers experience exposure to mixtures of genotoxicants in the workplace. BioMed Central 2011-03-24 /pmc/articles/PMC3071309/ /pubmed/21435260 http://dx.doi.org/10.1186/1476-069X-10-23 Text en Copyright ©2011 Al Zabadi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Al Zabadi, Hamzeh
Ferrari, Luc
Sari-Minodier, Irène
Kerautret, Marie-Aude
Tiberguent, Aziz
Paris, Christophe
Zmirou-Navier, Denis
Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
title Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
title_full Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
title_fullStr Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
title_full_unstemmed Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
title_short Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
title_sort integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071309/
https://www.ncbi.nlm.nih.gov/pubmed/21435260
http://dx.doi.org/10.1186/1476-069X-10-23
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