Cargando…
Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance
Most human somatic cells do not express telomerase. Consequently, with each cell division their telomeres progressively shorten until replicative senescence is induced. Approximately 15% of human cancers maintain their telomeres using telomerase-independent, recombination-based mechanisms collective...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071861/ https://www.ncbi.nlm.nih.gov/pubmed/21441915 http://dx.doi.org/10.1038/nsmb.2034 |
_version_ | 1782201483881611264 |
---|---|
author | Chang, Michael Dittmar, John C. Rothstein, Rodney |
author_facet | Chang, Michael Dittmar, John C. Rothstein, Rodney |
author_sort | Chang, Michael |
collection | PubMed |
description | Most human somatic cells do not express telomerase. Consequently, with each cell division their telomeres progressively shorten until replicative senescence is induced. Approximately 15% of human cancers maintain their telomeres using telomerase-independent, recombination-based mechanisms collectively termed Alternative Lengthening of Telomeres (ALT). In the yeast Saccharomyces cerevisiae, ALT cells are referred to as “survivors”. One type of survivor (type II) resembles human ALT cells in that both are defined by the amplification of telomeric repeats. We analyzed recombination-mediated telomere extension events at individual telomeres in telomerase-negative yeast during type II survivor formation and find that long telomeres are preferentially extended. Furthermore, we find that senescent cells with long telomeres are more efficient at bypassing senescence via the type II pathway. We speculate that telomere length may be important in determining whether cancer cells utilize telomerase or ALT to bypass replicative senescence. |
format | Text |
id | pubmed-3071861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
record_format | MEDLINE/PubMed |
spelling | pubmed-30718612011-10-01 Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance Chang, Michael Dittmar, John C. Rothstein, Rodney Nat Struct Mol Biol Article Most human somatic cells do not express telomerase. Consequently, with each cell division their telomeres progressively shorten until replicative senescence is induced. Approximately 15% of human cancers maintain their telomeres using telomerase-independent, recombination-based mechanisms collectively termed Alternative Lengthening of Telomeres (ALT). In the yeast Saccharomyces cerevisiae, ALT cells are referred to as “survivors”. One type of survivor (type II) resembles human ALT cells in that both are defined by the amplification of telomeric repeats. We analyzed recombination-mediated telomere extension events at individual telomeres in telomerase-negative yeast during type II survivor formation and find that long telomeres are preferentially extended. Furthermore, we find that senescent cells with long telomeres are more efficient at bypassing senescence via the type II pathway. We speculate that telomere length may be important in determining whether cancer cells utilize telomerase or ALT to bypass replicative senescence. 2011-03-27 2011-04 /pmc/articles/PMC3071861/ /pubmed/21441915 http://dx.doi.org/10.1038/nsmb.2034 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Chang, Michael Dittmar, John C. Rothstein, Rodney Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance |
title | Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance |
title_full | Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance |
title_fullStr | Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance |
title_full_unstemmed | Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance |
title_short | Long Telomeres are Preferentially Extended During Recombination-Mediated Telomere Maintenance |
title_sort | long telomeres are preferentially extended during recombination-mediated telomere maintenance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071861/ https://www.ncbi.nlm.nih.gov/pubmed/21441915 http://dx.doi.org/10.1038/nsmb.2034 |
work_keys_str_mv | AT changmichael longtelomeresarepreferentiallyextendedduringrecombinationmediatedtelomeremaintenance AT dittmarjohnc longtelomeresarepreferentiallyextendedduringrecombinationmediatedtelomeremaintenance AT rothsteinrodney longtelomeresarepreferentiallyextendedduringrecombinationmediatedtelomeremaintenance |