An effect of DNA sequence on nucleosome occupancy and removal

A barrier phases nucleosomes at the yeast (S. cerevisiae) GAL1/10 genes. Here we separate nucleosome positioning from occupancy, and show that the degree of occupancy of these phased sites is predictably determined by the underlying DNA sequences. As this occupancy is increased (by sequence alterati...

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Autores principales: Wang, Xin, Bryant, Gene O., Floer, Monique, Spagna, Dan, Ptashne, Mark
Formato: Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071895/
https://www.ncbi.nlm.nih.gov/pubmed/21378966
http://dx.doi.org/10.1038/nsmb.2017
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author Wang, Xin
Bryant, Gene O.
Floer, Monique
Spagna, Dan
Ptashne, Mark
author_facet Wang, Xin
Bryant, Gene O.
Floer, Monique
Spagna, Dan
Ptashne, Mark
author_sort Wang, Xin
collection PubMed
description A barrier phases nucleosomes at the yeast (S. cerevisiae) GAL1/10 genes. Here we separate nucleosome positioning from occupancy, and show that the degree of occupancy of these phased sites is predictably determined by the underlying DNA sequences. As this occupancy is increased (by sequence alteration) nucleosome removal upon induction is decreased, as is mRNA production. These results explain why promoter sequences have evolved to form nucleosomes relatively inefficiently.
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spelling pubmed-30718952011-10-01 An effect of DNA sequence on nucleosome occupancy and removal Wang, Xin Bryant, Gene O. Floer, Monique Spagna, Dan Ptashne, Mark Nat Struct Mol Biol Article A barrier phases nucleosomes at the yeast (S. cerevisiae) GAL1/10 genes. Here we separate nucleosome positioning from occupancy, and show that the degree of occupancy of these phased sites is predictably determined by the underlying DNA sequences. As this occupancy is increased (by sequence alteration) nucleosome removal upon induction is decreased, as is mRNA production. These results explain why promoter sequences have evolved to form nucleosomes relatively inefficiently. 2011-03-06 2011-04 /pmc/articles/PMC3071895/ /pubmed/21378966 http://dx.doi.org/10.1038/nsmb.2017 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Xin
Bryant, Gene O.
Floer, Monique
Spagna, Dan
Ptashne, Mark
An effect of DNA sequence on nucleosome occupancy and removal
title An effect of DNA sequence on nucleosome occupancy and removal
title_full An effect of DNA sequence on nucleosome occupancy and removal
title_fullStr An effect of DNA sequence on nucleosome occupancy and removal
title_full_unstemmed An effect of DNA sequence on nucleosome occupancy and removal
title_short An effect of DNA sequence on nucleosome occupancy and removal
title_sort effect of dna sequence on nucleosome occupancy and removal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3071895/
https://www.ncbi.nlm.nih.gov/pubmed/21378966
http://dx.doi.org/10.1038/nsmb.2017
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