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Optical properties of the mouse eye
The Shack-Hartmann wavefront sensor (SHWS) spots upon which ocular aberration measurements depend have poor quality in mice due to light reflected from multiple retinal layers. We have designed and implemented a SHWS that can favor light from a specific retinal layer and measured monochromatic aberr...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072116/ https://www.ncbi.nlm.nih.gov/pubmed/21483598 http://dx.doi.org/10.1364/BOE.2.000717 |
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author | Geng, Ying Schery, Lee Anne Sharma, Robin Dubra, Alfredo Ahmad, Kamran Libby, Richard T. Williams, David R. |
author_facet | Geng, Ying Schery, Lee Anne Sharma, Robin Dubra, Alfredo Ahmad, Kamran Libby, Richard T. Williams, David R. |
author_sort | Geng, Ying |
collection | PubMed |
description | The Shack-Hartmann wavefront sensor (SHWS) spots upon which ocular aberration measurements depend have poor quality in mice due to light reflected from multiple retinal layers. We have designed and implemented a SHWS that can favor light from a specific retinal layer and measured monochromatic aberrations in 20 eyes from 10 anesthetized C57BL/6J mice. Using this instrument, we show that mice are myopic, not hyperopic as is frequently reported. We have also measured longitudinal chromatic aberration (LCA) of the mouse eye and found that it follows predictions of the water-filled schematic mouse eye. Results indicate that the optical quality of the mouse eye assessed by measurement of its aberrations is remarkably good, better for retinal imaging than the human eye. The dilated mouse eye has a much larger numerical aperture (NA) than that of the dilated human eye (0.5 NA vs. 0.2 NA), but it has a similar amount of root mean square (RMS) higher order aberrations compared to the dilated human eye. These measurements predict that adaptive optics based on this method of wavefront sensing will provide improvements in retinal image quality and potentially two times higher lateral resolution than that in the human eye. |
format | Text |
id | pubmed-3072116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30721162011-04-11 Optical properties of the mouse eye Geng, Ying Schery, Lee Anne Sharma, Robin Dubra, Alfredo Ahmad, Kamran Libby, Richard T. Williams, David R. Biomed Opt Express Vision, Color, and Visual Optics The Shack-Hartmann wavefront sensor (SHWS) spots upon which ocular aberration measurements depend have poor quality in mice due to light reflected from multiple retinal layers. We have designed and implemented a SHWS that can favor light from a specific retinal layer and measured monochromatic aberrations in 20 eyes from 10 anesthetized C57BL/6J mice. Using this instrument, we show that mice are myopic, not hyperopic as is frequently reported. We have also measured longitudinal chromatic aberration (LCA) of the mouse eye and found that it follows predictions of the water-filled schematic mouse eye. Results indicate that the optical quality of the mouse eye assessed by measurement of its aberrations is remarkably good, better for retinal imaging than the human eye. The dilated mouse eye has a much larger numerical aperture (NA) than that of the dilated human eye (0.5 NA vs. 0.2 NA), but it has a similar amount of root mean square (RMS) higher order aberrations compared to the dilated human eye. These measurements predict that adaptive optics based on this method of wavefront sensing will provide improvements in retinal image quality and potentially two times higher lateral resolution than that in the human eye. Optical Society of America 2011-02-28 /pmc/articles/PMC3072116/ /pubmed/21483598 http://dx.doi.org/10.1364/BOE.2.000717 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Vision, Color, and Visual Optics Geng, Ying Schery, Lee Anne Sharma, Robin Dubra, Alfredo Ahmad, Kamran Libby, Richard T. Williams, David R. Optical properties of the mouse eye |
title | Optical properties of the mouse eye |
title_full | Optical properties of the mouse eye |
title_fullStr | Optical properties of the mouse eye |
title_full_unstemmed | Optical properties of the mouse eye |
title_short | Optical properties of the mouse eye |
title_sort | optical properties of the mouse eye |
topic | Vision, Color, and Visual Optics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072116/ https://www.ncbi.nlm.nih.gov/pubmed/21483598 http://dx.doi.org/10.1364/BOE.2.000717 |
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