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Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy
A custom built coherent anti-Stokes Raman scattering (CARS) microscope was used to image prostatic glands and nerve structures from 17 patients undergoing radical prostatectomy. Imaging of glandular and nerve structures showed distinctive cellular features that correlated to histological stains. Seg...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072130/ https://www.ncbi.nlm.nih.gov/pubmed/21483613 http://dx.doi.org/10.1364/BOE.2.000915 |
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author | Gao, Liang Zhou, Haijun Thrall, Michael J. Li, Fuhai Yang, Yaliang Wang, Zhiyong Luo, Pengfei Wong, Kelvin K. Palapattu, Ganesh S. Wong, Stephen T. C. |
author_facet | Gao, Liang Zhou, Haijun Thrall, Michael J. Li, Fuhai Yang, Yaliang Wang, Zhiyong Luo, Pengfei Wong, Kelvin K. Palapattu, Ganesh S. Wong, Stephen T. C. |
author_sort | Gao, Liang |
collection | PubMed |
description | A custom built coherent anti-Stokes Raman scattering (CARS) microscope was used to image prostatic glands and nerve structures from 17 patients undergoing radical prostatectomy. Imaging of glandular and nerve structures showed distinctive cellular features that correlated to histological stains. Segmentation of cell nucleus was performed to establish a cell feature-based model to separate normal glands from cancer glands. In this study, we use a single parameter, average cell neighbor distance based on CARS imaging, to characterize normal and cancerous glandular structures. By combining CARS with our novel classification model, we are able to characterize prostate glandular and nerve structures in a manner that potentially enables real-time, intra-operative assessment of surgical margins and neurovascular bundles. As such, this method could potentially improve outcomes following radical prostatectomy. |
format | Text |
id | pubmed-3072130 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30721302011-04-11 Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy Gao, Liang Zhou, Haijun Thrall, Michael J. Li, Fuhai Yang, Yaliang Wang, Zhiyong Luo, Pengfei Wong, Kelvin K. Palapattu, Ganesh S. Wong, Stephen T. C. Biomed Opt Express Optics in Cancer Research A custom built coherent anti-Stokes Raman scattering (CARS) microscope was used to image prostatic glands and nerve structures from 17 patients undergoing radical prostatectomy. Imaging of glandular and nerve structures showed distinctive cellular features that correlated to histological stains. Segmentation of cell nucleus was performed to establish a cell feature-based model to separate normal glands from cancer glands. In this study, we use a single parameter, average cell neighbor distance based on CARS imaging, to characterize normal and cancerous glandular structures. By combining CARS with our novel classification model, we are able to characterize prostate glandular and nerve structures in a manner that potentially enables real-time, intra-operative assessment of surgical margins and neurovascular bundles. As such, this method could potentially improve outcomes following radical prostatectomy. Optical Society of America 2011-03-18 /pmc/articles/PMC3072130/ /pubmed/21483613 http://dx.doi.org/10.1364/BOE.2.000915 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Optics in Cancer Research Gao, Liang Zhou, Haijun Thrall, Michael J. Li, Fuhai Yang, Yaliang Wang, Zhiyong Luo, Pengfei Wong, Kelvin K. Palapattu, Ganesh S. Wong, Stephen T. C. Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy |
title | Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy |
title_full | Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy |
title_fullStr | Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy |
title_full_unstemmed | Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy |
title_short | Label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-Stokes Raman scattering microscopy |
title_sort | label-free high-resolution imaging of prostate glands and cavernous nerves using coherent anti-stokes raman scattering microscopy |
topic | Optics in Cancer Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072130/ https://www.ncbi.nlm.nih.gov/pubmed/21483613 http://dx.doi.org/10.1364/BOE.2.000915 |
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