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Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy
We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bu...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072136/ https://www.ncbi.nlm.nih.gov/pubmed/21483619 http://dx.doi.org/10.1364/BOE.2.000981 |
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author | Elahi, Sakib F. Miller, Sharon J. Joshi, Bishnu Wang, Thomas D. |
author_facet | Elahi, Sakib F. Miller, Sharon J. Joshi, Bishnu Wang, Thomas D. |
author_sort | Elahi, Sakib F. |
collection | PubMed |
description | We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bundle with outer diameter of 680 µm to collect en face images at 10 Hz with 4 µm lateral resolution. We applied the FITC-labeled peptide QPIHPNNM topically to colonic mucosa in genetically engineered mice that spontaneously develop adenomas. More than two-fold greater fluorescence intensity was measured from adenomas compared to adjacent normal-appearing mucosa. Images of adenomas showed irregular morphology characteristic of dysplasia. |
format | Text |
id | pubmed-3072136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-30721362011-04-11 Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy Elahi, Sakib F. Miller, Sharon J. Joshi, Bishnu Wang, Thomas D. Biomed Opt Express Endoscopes, Catheters and Micro-Optics We validate specific binding activity of a fluorescence-labeled peptide to colorectal dysplasia in living mice using a miniature, flexible, fiber microendoscope that passes through the instrument channel of an endoscope. The microendoscope delivers excitation light at 473 nm through a fiber-optic bundle with outer diameter of 680 µm to collect en face images at 10 Hz with 4 µm lateral resolution. We applied the FITC-labeled peptide QPIHPNNM topically to colonic mucosa in genetically engineered mice that spontaneously develop adenomas. More than two-fold greater fluorescence intensity was measured from adenomas compared to adjacent normal-appearing mucosa. Images of adenomas showed irregular morphology characteristic of dysplasia. Optical Society of America 2011-03-28 /pmc/articles/PMC3072136/ /pubmed/21483619 http://dx.doi.org/10.1364/BOE.2.000981 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Endoscopes, Catheters and Micro-Optics Elahi, Sakib F. Miller, Sharon J. Joshi, Bishnu Wang, Thomas D. Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
title | Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
title_full | Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
title_fullStr | Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
title_full_unstemmed | Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
title_short | Targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
title_sort | targeted imaging of colorectal dysplasia in living mice with fluorescence microendoscopy |
topic | Endoscopes, Catheters and Micro-Optics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072136/ https://www.ncbi.nlm.nih.gov/pubmed/21483619 http://dx.doi.org/10.1364/BOE.2.000981 |
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